Detailed information of TA system TA076493

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 27266..27692
• Replicon: plasmid p95JB1B
• Accession: NZ_CP021337
• Organism: Escherichia coli strain 95JB1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: EC95JB1_RS29225
• Protein ID: WP_001312861.1
• Coordinates: 27266..27424 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	27468..27692 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC95JB1_RS29185	22637..23326	-	690	WP_000283373.1	conjugal transfer transcriptional regulator TraJ	-
EC95JB1_RS29190	23513..23896	-	384	WP_001151524.1	relaxosome protein TraM	-
EC95JB1_RS29195	24310..24819	+	510	Protein_23	transglycosylase SLT domain-containing protein	-
EC95JB1_RS29200	25116..25937	-	822	WP_001234469.1	DUF945 domain-containing protein	-
EC95JB1_RS29205	26057..26344	-	288	WP_000107535.1	hypothetical protein	-
EC95JB1_RS29225	27266..27424	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	27468..27692	-	225	NuclAT_0	-	Antitoxin
-	27468..27692	-	225	NuclAT_0	-	Antitoxin
-	27468..27692	-	225	NuclAT_0	-	Antitoxin
-	27468..27692	-	225	NuclAT_0	-	Antitoxin
EC95JB1_RS30135	27504..27692	+	189	WP_001299721.1	hypothetical protein	-
EC95JB1_RS29230	27704..28423	-	720	WP_001276234.1	plasmid SOS inhibition protein A	-
EC95JB1_RS29235	28420..28854	-	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
EC95JB1_RS29240	28909..30867	-	1959	WP_001145473.1	ParB/RepB/Spo0J family partition protein	-
EC95JB1_RS29245	30926..31159	-	234	WP_000006004.1	DUF905 domain-containing protein	-
EC95JB1_RS29250	31215..31742	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
EC95JB1_RS29270	32215..32475	-	261	WP_071600428.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espP / hlyC / hlyA / hlyB / hlyD	1..83138	83138

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T76493 WP_001312861.1 NZ_CP021337:c27424-27266 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T76493 NZ_CP021337:c27424-27266 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT76493 NZ_CP021337:c27692-27468 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin