Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2493989..2494491 Replicon chromosome
Accession NZ_CP021335
Organism Escherichia coli strain 95JB1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EC95JB1_RS13650 Protein ID WP_000767829.1
Coordinates 2493989..2494243 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EC95JB1_RS13655 Protein ID WP_001259255.1
Coordinates 2494240..2494491 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC95JB1_RS13625 2489004..2489231 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
EC95JB1_RS13630 2489245..2490303 - 1059 WP_000492340.1 transport protein YeeE -
EC95JB1_RS13635 2490482..2491840 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EC95JB1_RS29875 2491830..2491892 - 63 WP_010723108.1 membrane protein YoeI -
EC95JB1_RS13640 2492107..2493036 - 930 WP_000803331.1 LysR family transcriptional regulator -
EC95JB1_RS13645 2493082..2493906 - 825 WP_000754755.1 SDR family oxidoreductase -
EC95JB1_RS13650 2493989..2494243 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EC95JB1_RS13655 2494240..2494491 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EC95JB1_RS13660 2494775..2494825 + 51 WP_001364200.1 his operon leader peptide -
EC95JB1_RS13670 2494971..2495870 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
EC95JB1_RS13675 2495876..2497180 + 1305 WP_001345258.1 histidinol dehydrogenase -
EC95JB1_RS13680 2497177..2498256 + 1080 WP_000102292.1 histidinol-phosphate transaminase -
EC95JB1_RS13685 2498253..2499320 + 1068 WP_000080054.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T76482 WP_000767829.1 NZ_CP021335:c2494243-2493989 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T76482 NZ_CP021335:c2494243-2493989 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT76482 WP_001259255.1 NZ_CP021335:c2494491-2494240 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT76482 NZ_CP021335:c2494491-2494240 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References