Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 280192..280733 Replicon chromosome
Accession NZ_CP021335
Organism Escherichia coli strain 95JB1

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag EC95JB1_RS01310 Protein ID WP_000615976.1
Coordinates 280192..280470 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag EC95JB1_RS01315 Protein ID WP_000729704.1
Coordinates 280473..280733 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC95JB1_RS01295 277774..278352 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
EC95JB1_RS01300 278558..279325 + 768 WP_000333380.1 class II glutamine amidotransferase -
EC95JB1_RS01305 279296..280036 - 741 WP_001225679.1 murein L,D-transpeptidase -
EC95JB1_RS01310 280192..280470 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EC95JB1_RS01315 280473..280733 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EC95JB1_RS01320 280919..281692 + 774 Protein_246 C40 family peptidase -
EC95JB1_RS01325 281749..282105 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
EC95JB1_RS01330 282098..282376 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
EC95JB1_RS01335 282481..284574 - 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
EC95JB1_RS01340 284558..285697 - 1140 WP_000785663.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 271064..282376 11312


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T76468 WP_000615976.1 NZ_CP021335:c280470-280192 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T76468 NZ_CP021335:c280470-280192 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT76468 WP_000729704.1 NZ_CP021335:c280733-280473 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT76468 NZ_CP021335:c280733-280473 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References