Detailed information of TA system TA076435

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 94814..95240
• Replicon: plasmid pPA45B
• Accession: NZ_CP021289
• Organism: Escherichia coli strain PA45B

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: PA45B_RS26865
• Protein ID: WP_001312861.1
• Coordinates: 94814..94972 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	95016..95240 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA45B_RS26830	90211..90900	-	690	WP_000283387.1	conjugal transfer transcriptional regulator TraJ	-
PA45B_RS26835	91087..91470	-	384	WP_001151538.1	relaxosome protein TraM	-
PA45B_RS26840	91791..92393	+	603	Protein_104	transglycosylase SLT domain-containing protein	-
PA45B_RS26845	92690..93511	-	822	WP_001234445.1	DUF945 domain-containing protein	-
PA45B_RS26850	93621..93917	-	297	WP_001545326.1	hypothetical protein	-
PA45B_RS27660	94309..94513	+	205	Protein_107	pilus protein	-
PA45B_RS26865	94814..94972	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	95016..95240	-	225	NuclAT_0	-	Antitoxin
-	95016..95240	-	225	NuclAT_0	-	Antitoxin
-	95016..95240	-	225	NuclAT_0	-	Antitoxin
-	95016..95240	-	225	NuclAT_0	-	Antitoxin
PA45B_RS26870	95052..95231	+	180	WP_001309233.1	hypothetical protein	-
PA45B_RS26875	95252..95971	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
PA45B_RS26880	95968..96402	-	435	WP_021529837.1	conjugation system SOS inhibitor PsiB	-
PA45B_RS26885	96471..98435	-	1965	WP_001537566.1	ParB/RepB/Spo0J family partition protein	-
PA45B_RS26890	98496..98729	-	234	WP_000006003.1	DUF905 family protein	-
PA45B_RS26895	98787..99314	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / qacE / sul1 / blaTEM-1B	senB	1..147172	147172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T76435 WP_001312861.1 NZ_CP021289:c94972-94814 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T76435 NZ_CP021289:c94972-94814 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT76435 NZ_CP021289:c95240-95016 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin