Detailed information of TA system TA076431

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 52528..52781
• Replicon: plasmid pPA45B
• Accession: NZ_CP021289
• Organism: Escherichia coli strain PA45B

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: PA45B_RS26595
• Protein ID: WP_001336447.1
• Coordinates: 52528..52677 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	52725..52781 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA45B_RS26550	47587..48284	-	698	Protein_50	IS1 family transposase	-
PA45B_RS26560	48552..48953	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
PA45B_RS27655	48886..49143	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
PA45B_RS26565	49236..49889	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
PA45B_RS27840	49987..50127	-	141	WP_198319705.1	hypothetical protein	-
PA45B_RS26575	50828..51685	-	858	WP_001537577.1	incFII family plasmid replication initiator RepA	-
PA45B_RS26580	51678..51752	-	75	WP_001365705.1	RepA leader peptide Tap	-
PA45B_RS26590	51986..52243	-	258	WP_000084404.1	replication regulatory protein RepA	-
PA45B_RS26595	52528..52677	-	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	52725..52781	+	57	NuclAT_1	-	Antitoxin
-	52725..52781	+	57	NuclAT_1	-	Antitoxin
-	52725..52781	+	57	NuclAT_1	-	Antitoxin
-	52725..52781	+	57	NuclAT_1	-	Antitoxin
PA45B_RS26605	52876..53313	-	438	WP_000872609.1	hypothetical protein	-
PA45B_RS26610	53466..53849	-	384	WP_001109264.1	hypothetical protein	-
PA45B_RS26615	53952..54413	-	462	WP_000760080.1	thermonuclease family protein	-
PA45B_RS26620	55166..55369	-	204	WP_001336517.1	hypothetical protein	-
PA45B_RS26625	55521..56078	-	558	WP_000139329.1	fertility inhibition protein FinO	-
PA45B_RS26630	56133..56879	-	747	WP_000205709.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / qacE / sul1 / blaTEM-1B	senB	1..147172	147172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T76431 WP_001336447.1 NZ_CP021289:c52677-52528 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T76431 NZ_CP021289:c52677-52528 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT76431 NZ_CP021289:52725-52781 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin