Detailed information of TA system TA076425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4376248..4376850
• Replicon: chromosome
• Accession: NZ_CP021288
• Organism: Escherichia coli strain PA45B

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: PA45B_RS22600
• Protein ID: WP_000897302.1
• Coordinates: 4376248..4376559 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PA45B_RS22605
• Protein ID: WP_000356397.1
• Coordinates: 4376560..4376850 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA45B_RS22575	4372162..4372761	+	600	WP_001314324.1	glucose-1-phosphatase	-
PA45B_RS22580	4372755..4373627	+	873	WP_000920762.1	virulence factor BrkB family protein	-
PA45B_RS22585	4373624..4374061	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
PA45B_RS22590	4374106..4375047	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
PA45B_RS22595	4375111..4376019	-	909	WP_001331553.1	alpha/beta hydrolase	-
PA45B_RS22600	4376248..4376559	+	312	WP_000897302.1	hypothetical protein	Toxin
PA45B_RS22605	4376560..4376850	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
PA45B_RS22615	4377209..4377487	+	279	WP_001296612.1	hypothetical protein	-
PA45B_RS22625	4377883..4378101	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
PA45B_RS22630	4378317..4379246	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
PA45B_RS22635	4379243..4379878	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
PA45B_RS22640	4379875..4380777	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T76425 WP_000897302.1 NZ_CP021288:4376248-4376559 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T76425 NZ_CP021288:4376248-4376559 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT76425 WP_000356397.1 NZ_CP021288:4376560-4376850 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT76425 NZ_CP021288:4376560-4376850 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416