Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2180033..2180535 Replicon chromosome
Accession NZ_CP021288
Organism Escherichia coli strain PA45B

Toxin (Protein)


Gene name yoeB Uniprot ID A0A0H2Z0A6
Locus tag PA45B_RS11425 Protein ID WP_000767826.1
Coordinates 2180033..2180287 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag PA45B_RS11430 Protein ID WP_001259255.1
Coordinates 2180284..2180535 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PA45B_RS11410 2176526..2177884 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
PA45B_RS27470 2177874..2177936 - 63 WP_010723108.1 membrane protein YoeI -
PA45B_RS11415 2178151..2179080 - 930 WP_000803363.1 LysR family transcriptional regulator -
PA45B_RS11420 2179126..2179950 - 825 WP_000754737.1 SDR family oxidoreductase -
PA45B_RS11425 2180033..2180287 - 255 WP_000767826.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
PA45B_RS11430 2180284..2180535 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
PA45B_RS11435 2180818..2180868 + 51 WP_001364200.1 his operon leader peptide -
PA45B_RS11440 2181014..2181913 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
PA45B_RS11445 2181919..2183223 + 1305 WP_001350692.1 histidinol dehydrogenase -
PA45B_RS11450 2183220..2184290 + 1071 WP_000108997.1 histidinol-phosphate transaminase -
PA45B_RS11455 2184290..2185357 + 1068 WP_000080128.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10195.64 Da        Isoelectric Point: 8.0353

>T76421 WP_000767826.1 NZ_CP021288:c2180287-2180033 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRIIEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T76421 NZ_CP021288:c2180287-2180033 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTATAGAGGAACACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT76421 WP_001259255.1 NZ_CP021288:c2180535-2180284 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT76421 NZ_CP021288:c2180535-2180284 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCACCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2Z0A6

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References