Detailed information of TA system TA076403

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 284002..284696
• Replicon: chromosome
• Accession: NZ_CP021288
• Organism: Escherichia coli strain PA45B

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: PA45B_RS01360
• Protein ID: WP_001263500.1
• Coordinates: 284298..284696 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: PA45B_RS01355
• Protein ID: WP_000554758.1
• Coordinates: 284002..284295 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA45B_RS01335	279538..280035	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
PA45B_RS27265	280118..280276	-	159	WP_014639450.1	hypothetical protein	-
PA45B_RS01340	280355..282067	-	1713	Protein_253	flagellar biosynthesis protein FlhA	-
PA45B_RS01345	282039..282824	+	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
PA45B_RS01350	282895..283950	+	1056	WP_001226168.1	DNA polymerase IV	-
PA45B_RS01355	284002..284295	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
PA45B_RS01360	284298..284696	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
PA45B_RS01365	284706..285158	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
PA45B_RS01370	285348..286487	+	1140	WP_000521577.1	RNA ligase RtcB family protein	-
PA45B_RS01375	286484..287098	+	615	WP_000602123.1	peptide chain release factor H	-
PA45B_RS01380	287155..288612	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
PA45B_RS01385	288873..289331	+	459	WP_001291988.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	264477..285158	20681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T76403 WP_001263500.1 NZ_CP021288:284298-284696 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T76403 NZ_CP021288:284298-284696 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT76403 WP_000554758.1 NZ_CP021288:284002-284295 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT76403 NZ_CP021288:284002-284295 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3