Detailed information of TA system TA076398

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_31
• Location: 74430..75034
• Replicon: chromosome
• Accession: NZ_CP021286
• Organism: Legionella pneumophila subsp. pneumophila strain Albuquerque

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A128WF47
• Locus tag: CA919_RS00385
• Protein ID: WP_011212715.1
• Coordinates: 74430..74753 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CA919_RS00390
• Protein ID: WP_011212716.1
• Coordinates: 74750..75034 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA919_RS00360	69790..70635	-	846	WP_011212710.1	hypothetical protein	-
CA919_RS00365	70964..71218	-	255	WP_041173947.1	hypothetical protein	-
CA919_RS00370	71756..72697	+	942	WP_011212712.1	hypothetical protein	-
CA919_RS00375	72706..72801	+	96	Protein_71	transposase	-
CA919_RS00380	73126..74346	+	1221	WP_011212714.1	site-specific integrase	-
CA919_RS00385	74430..74753	+	324	WP_011212715.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CA919_RS00390	74750..75034	+	285	WP_011212716.1	helix-turn-helix domain-containing protein	Antitoxin
CA919_RS00395	75216..76049	+	834	WP_011212717.1	hypothetical protein	-
CA919_RS00400	76355..76753	+	399	WP_011212718.1	DUF1780 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-86)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12144.36 Da
• Isoelectric Point: 9.7878

>T76398 WP_011212715.1 NZ_CP021286:74430-74753 [Legionella pneumophila subsp. pneumophila]
MSWIVEFYNESVEEAILTMPPKIQARMIKLLELIETHGANLGPPHTEAMGDGLFEIRAKAQEGIGRSLYCYMKGKHIVVL
HAFVKKSAKTPKPDLQLALKRKREVEK

Nucleotide

• Length: 324 bp

>T76398 NZ_CP021286:74430-74753 [Legionella pneumophila subsp. pneumophila]
ATGTCTTGGATTGTTGAATTTTATAATGAGTCTGTTGAAGAGGCCATACTTACAATGCCGCCAAAAATTCAGGCTCGAAT
GATTAAACTTTTGGAATTGATTGAAACACATGGCGCTAATCTTGGGCCTCCTCATACAGAAGCGATGGGTGATGGGTTAT
TTGAGATTCGCGCAAAAGCTCAAGAAGGAATCGGGCGATCGCTTTATTGCTATATGAAGGGAAAGCATATTGTGGTTTTG
CATGCATTTGTAAAGAAAAGTGCAAAAACTCCAAAACCAGATTTGCAATTAGCTCTCAAAAGAAAGCGGGAGGTAGAAAA
ATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.22 Da
• Isoelectric Point: 9.8787

>AT76398 WP_011212716.1 NZ_CP021286:74750-75034 [Legionella pneumophila subsp. pneumophila]
MMKLKTLKAKALKNPEVKEAYDALESEFALIDALLTMRQKAGLTQEELAERMGTKKGNISRLEKGNTNPSWNTLEKYAHA
CGFDISMAVKRIAS

Nucleotide

• Length: 285 bp

>AT76398 NZ_CP021286:74750-75034 [Legionella pneumophila subsp. pneumophila]
ATGATGAAACTTAAAACATTAAAGGCTAAAGCGTTAAAAAATCCTGAAGTAAAAGAAGCATACGATGCTTTGGAATCCGA
ATTTGCATTGATCGATGCGTTACTGACGATGCGTCAAAAAGCTGGATTAACTCAAGAAGAGTTAGCTGAGCGAATGGGGA
CAAAAAAAGGGAATATTTCTCGACTGGAAAAAGGAAATACTAATCCAAGCTGGAATACTTTAGAGAAATATGCACATGCG
TGTGGGTTTGATATTTCAATGGCAGTAAAGAGAATCGCTTCATAA

Structures

Toxin

• AlphaFold DB: A0A128WF47