Detailed information of TA system TA076364

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 2653795..2655038
• Replicon: chromosome
• Accession: NZ_CP021266
• Organism: Legionella pneumophila subsp. pneumophila strain Knoxville

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: CA925_RS11950
• Protein ID: WP_027224015.1
• Coordinates: 2654100..2655038 (+)
• Length: 313 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: A0A1E5JNB7
• Locus tag: CA925_RS11945
• Protein ID: WP_027224016.1
• Coordinates: 2653795..2654103 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA925_RS11925	2649322..2650446	-	1125	WP_027224020.1	UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)	-
CA925_RS11930	2650822..2652573	-	1752	WP_027224019.1	DUF927 domain-containing protein	-
CA925_RS11935	2652657..2652932	-	276	WP_027224018.1	helix-turn-helix domain-containing protein	-
CA925_RS11940	2653571..2653798	+	228	WP_027224017.1	helix-turn-helix transcriptional regulator	-
CA925_RS11945	2653795..2654103	+	309	WP_027224016.1	HipA N-terminal domain-containing protein	Antitoxin
CA925_RS11950	2654100..2655038	+	939	WP_027224015.1	lpg2370 family Dot/Icm T4SS effector	Toxin
CA925_RS11955	2655035..2655226	-	192	WP_032829667.1	hypothetical protein	-
CA925_RS11960	2655328..2655753	-	426	WP_027224014.1	helix-turn-helix domain-containing protein	-
CA925_RS11965	2655756..2656043	-	288	WP_027224013.1	type II toxin-antitoxin system HigB family toxin	-
CA925_RS11970	2656107..2657336	-	1230	WP_027224012.1	integrase family protein	-
CA925_RS11975	2657581..2658567	-	987	WP_042236413.1	IS30 family transposase	-
CA925_RS11980	2658749..2659537	+	789	WP_014844522.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	lpg2370	2635515..2686180	50665

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (53-276)

Antitoxin

• HipA, N-terminal subdomain 1 (5-98)

Sequences

Toxin

Protein

• Length: 313 a.a.
• Molecular weight: 36029.85 Da
• Isoelectric Point: 9.1296

>T76364 WP_027224015.1 NZ_CP021266:2654100-2655038 [Legionella pneumophila subsp. pneumophila]
MKHCPITYEKISVQENYSQRGLHLLSPQLKNLSPLDLSADEQRQEAIARVGKMSVQGVQKKLSAKLKIKEGCFEIVDQYG
QYILKPQSDIYPELPENEAITMTLAKTIGLEVPVHGLVYSKDNSLTYFIKRFDRIGHNKKLALEDFAQLSGEDRHTKYKS
SMEKVIAVIEQFCTFPKIEFVKLFKLTLFNFLVGNEDMHLKNFSLITKDRKISISPAYDLLNSTIAQKNTKEELALPLKG
KKNNLTKSDFLKYFAIEKLGLNQNVIDGIVQEFHQVIPKWQELIGFSFLSQPMQEKYLELLEQRCKRLNFFD

Nucleotide

• Length: 939 bp

>T76364 NZ_CP021266:2654100-2655038 [Legionella pneumophila subsp. pneumophila]
ATGAAACACTGTCCTATTACTTATGAAAAGATAAGTGTTCAAGAAAACTATTCGCAACGTGGATTGCATTTGCTTTCTCC
TCAATTAAAAAATCTTAGCCCATTAGATTTAAGTGCTGATGAGCAGCGACAGGAAGCAATTGCTCGTGTAGGAAAGATGT
CTGTTCAGGGCGTCCAAAAGAAACTAAGTGCCAAACTAAAGATTAAAGAGGGATGTTTTGAAATCGTTGATCAATATGGT
CAGTATATTTTAAAACCACAAAGTGATATTTATCCAGAATTGCCTGAAAATGAAGCCATTACTATGACCCTTGCAAAAAC
TATAGGCCTCGAAGTTCCGGTTCATGGTTTGGTTTATTCTAAAGACAACAGTTTAACCTACTTCATTAAACGCTTTGATA
GAATAGGCCATAATAAAAAGTTGGCTTTAGAAGATTTTGCACAGCTATCAGGTGAAGATCGACATACAAAATATAAAAGT
TCTATGGAAAAAGTAATTGCAGTCATAGAACAGTTTTGTACTTTCCCAAAAATTGAATTCGTGAAATTATTTAAGTTGAC
GTTGTTTAACTTCCTGGTCGGCAATGAAGACATGCATCTAAAAAATTTTTCCTTAATTACTAAGGATAGAAAAATTTCCA
TATCACCAGCATATGATTTACTTAACTCAACTATCGCTCAAAAAAATACCAAAGAAGAACTGGCTTTACCTCTAAAAGGA
AAAAAAAATAATTTAACAAAGAGTGATTTTCTTAAATATTTTGCCATTGAAAAATTAGGATTAAATCAGAACGTTATCGA
TGGGATCGTACAAGAGTTCCATCAGGTAATACCAAAATGGCAAGAGCTGATTGGTTTCAGTTTTTTATCTCAACCGATGC
AGGAAAAATATCTCGAGTTGCTAGAGCAACGGTGCAAGCGATTAAATTTTTTTGATTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11626.49 Da
• Isoelectric Point: 4.6017

>AT76364 WP_027224016.1 NZ_CP021266:2653795-2654103 [Legionella pneumophila subsp. pneumophila]
MRKAYVSVNGIRAGILEELEGKKYQFTYLNDYQGAPVSLTMPLTNKVYDFDVFPPFFEGLLPEGIMLEALLRKYKIDKND
YFGQLILVGQDVVGAVTIEELR

Nucleotide

• Length: 309 bp

>AT76364 NZ_CP021266:2653795-2654103 [Legionella pneumophila subsp. pneumophila]
ATGAGAAAAGCATACGTATCAGTCAATGGCATTAGAGCTGGAATATTAGAGGAGTTAGAAGGTAAAAAATATCAATTTAC
TTACCTTAATGATTATCAGGGGGCACCTGTTTCTCTCACCATGCCGTTAACCAATAAGGTCTATGATTTTGACGTGTTTC
CCCCTTTTTTTGAAGGATTACTTCCTGAAGGCATTATGCTTGAAGCACTATTACGCAAATATAAAATCGATAAAAATGAT
TATTTTGGCCAATTGATACTAGTTGGCCAAGATGTCGTAGGCGCAGTGACGATTGAGGAACTAAGATGA

Structures

Antitoxin

• AlphaFold DB: A0A1E5JNB7