Detailed information of TA system TA076350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 2697655..2698898
• Replicon: chromosome
• Accession: NZ_CP021259
• Organism: Legionella pneumophila strain D-7708

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: CA934_RS12225
• Protein ID: WP_106225652.1
• Coordinates: 2697960..2698898 (+)
• Length: 313 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: A0A2S6EX97
• Locus tag: CA934_RS12220
• Protein ID: WP_027227416.1
• Coordinates: 2697655..2697963 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA934_RS12185	2693004..2693864	+	861	WP_106225651.1	DUF2608 domain-containing protein	-
CA934_RS12190	2694216..2694480	-	265	Protein_2362	hypothetical protein	-
CA934_RS12195	2694528..2694839	-	312	WP_027227411.1	cupin	-
CA934_RS12200	2694855..2695103	-	249	WP_031301595.1	hypothetical protein	-
CA934_RS12205	2695410..2695883	+	474	WP_027227413.1	hypothetical protein	-
CA934_RS12210	2696073..2696990	+	918	WP_027227414.1	LysR family transcriptional regulator	-
CA934_RS12215	2697431..2697658	+	228	WP_027227415.1	helix-turn-helix transcriptional regulator	-
CA934_RS12220	2697655..2697963	+	309	WP_027227416.1	HipA N-terminal domain-containing protein	Antitoxin
CA934_RS12225	2697960..2698898	+	939	WP_106225652.1	lpg2370 family Dot/Icm T4SS effector	Toxin
CA934_RS12230	2699195..2700358	+	1164	WP_106225653.1	serine kinase	-
CA934_RS12235	2700350..2701537	-	1188	WP_106225654.1	fatty acid desaturase	-
CA934_RS12240	2701662..2701829	-	168	WP_011214444.1	DUF3309 domain-containing protein	-
CA934_RS12245	2702428..2702784	+	357	WP_063720028.1	hypothetical protein	-
CA934_RS12250	2702825..2703757	-	933	WP_106225655.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (53-275)

Antitoxin

• HipA, N-terminal subdomain 1 (3-98)

Sequences

Toxin

Protein

• Length: 313 a.a.
• Molecular weight: 36124.96 Da
• Isoelectric Point: 9.5798

>T76350 WP_106225652.1 NZ_CP021259:2697960-2698898 [Legionella pneumophila]
MKRCPITYERISDQENYSQRGLHLLSPQLKNLSPLDLSADEQRQEAIARVGKMSIQGVQKKLSAKLKIKEGCFEIVDQNG
HYILKPQSDIYPELPENEAITMTLAKTIGLEVPVHGLVYSKDNSLTYFIKRFDRIGHNKKLALEDFAQLSGEDRHTKYKS
SMEKVIAVVEQFCTFPKIEFVKLFKLTLFNFLVGNEDMHLKNFSLITKDRKISISPAYDLLNSTIAQKNTKEEIALPLKG
KKNNLTKSDFLKYFAIEKLGLNQKVIDGIVQEFHQIIPEWRKLVGFSFLSQQMQEKYLQLLEQRCKRLNFFD

Nucleotide

• Length: 939 bp

>T76350 NZ_CP021259:2697960-2698898 [Legionella pneumophila]
ATGAAACGCTGTCCTATTACTTATGAAAGGATTAGTGACCAGGAAAACTACTCGCAACGTGGATTGCATTTGCTTTCTCC
TCAATTAAAAAATCTTAGCCCATTAGATTTAAGTGCTGATGAGCAGCGTCAGGAAGCAATTGCTCGAGTAGGAAAGATGT
CTATTCAAGGTGTCCAAAAGAAACTAAGCGCAAAACTAAAGATTAAAGAGGGATGTTTTGAAATTGTGGATCAAAATGGC
CACTATATTTTAAAACCACAAAGTGATATTTATCCAGAATTGCCGGAAAATGAAGCCATTACTATGACCCTAGCAAAAAC
CATTGGCCTTGAAGTTCCGGTTCATGGTTTAGTTTATTCTAAAGACAACAGTTTGACCTACTTTATTAAACGGTTTGATC
GAATAGGCCATAATAAAAAGTTAGCTTTAGAAGATTTTGCACAGCTATCAGGTGAAGATCGACATACAAAATATAAAAGT
TCTATGGAAAAAGTCATTGCAGTCGTAGAACAGTTTTGTACATTCCCCAAAATTGAATTCGTGAAATTATTTAAGTTGAC
GTTATTTAATTTCCTGGTCGGCAATGAAGACATGCATCTAAAAAATTTTTCCCTAATTACTAAGGATAGAAAAATTTCCA
TATCACCAGCCTATGATTTACTTAACTCAACTATTGCTCAAAAAAACACAAAAGAAGAAATAGCCTTGCCTCTAAAAGGG
AAAAAAAATAATTTAACAAAGAGTGATTTTCTTAAATACTTCGCCATTGAAAAATTAGGATTAAATCAGAAAGTCATCGA
TGGGATCGTACAAGAGTTCCATCAAATAATACCTGAATGGCGAAAGCTGGTTGGTTTCAGTTTTTTATCTCAACAAATGC
AAGAAAAATATCTCCAATTGCTAGAGCAACGATGCAAGCGATTAAATTTTTTTGATTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11611.39 Da
• Isoelectric Point: 4.5556

>AT76350 WP_027227416.1 NZ_CP021259:2697655-2697963 [Legionella pneumophila]
MRKAYVSVNGIKAGTLEELQGGKYQFTYFDDYQGAPVSLTMPLTNKVYYFDVFPPFFEGLLPEGIMLEALLRKYKIDRND
YFGQLVIVGQDVVGAVTIEELR

Nucleotide

• Length: 309 bp

>AT76350 NZ_CP021259:2697655-2697963 [Legionella pneumophila]
ATGAGAAAAGCATACGTATCAGTGAATGGCATTAAGGCTGGAACATTAGAAGAATTACAAGGTGGAAAATATCAATTTAC
CTACTTTGATGATTATCAGGGCGCGCCTGTTTCTCTCACAATGCCGTTAACCAATAAGGTCTATTATTTTGACGTATTCC
CTCCTTTCTTTGAAGGATTACTTCCTGAAGGCATTATGCTTGAAGCACTATTACGTAAATATAAAATTGATAGAAATGAC
TATTTTGGTCAATTGGTAATAGTTGGCCAAGATGTCGTAGGCGCAGTGACTATTGAGGAACTAAGATGA

Structures

Antitoxin

• AlphaFold DB: A0A2S6EX97