Detailed information of TA system TA076339

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_31
• Location: 68475..69079
• Replicon: chromosome
• Accession: NZ_CP021256
• Organism: Legionella pneumophila strain D-4954

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A128WF47
• Locus tag: CA937_RS00325
• Protein ID: WP_011212715.1
• Coordinates: 68475..68798 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CA937_RS00330
• Protein ID: WP_011212716.1
• Coordinates: 68795..69079 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA937_RS00300	63554..63772	+	219	WP_062726833.1	hypothetical protein	-
CA937_RS00305	63943..64431	+	489	WP_027228350.1	YcxB family protein	-
CA937_RS00310	64793..66205	-	1413	WP_106184409.1	hypothetical protein	-
CA937_RS00315	66236..66844	-	609	WP_062726835.1	hypothetical protein	-
CA937_RS00320	67171..68391	+	1221	WP_040147087.1	site-specific integrase	-
CA937_RS00325	68475..68798	+	324	WP_011212715.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CA937_RS00330	68795..69079	+	285	WP_011212716.1	helix-turn-helix domain-containing protein	Antitoxin
CA937_RS00335	69261..70094	+	834	WP_040147091.1	hypothetical protein	-
CA937_RS00340	70350..70703	+	354	WP_027225314.1	DUF2513 domain-containing protein	-
CA937_RS00345	71261..71953	+	693	WP_040147093.1	hypothetical protein	-
CA937_RS00350	72239..72457	+	219	WP_027225316.1	hypothetical protein	-
CA937_RS00355	72460..72918	-	459	WP_027225317.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	67171..76428	9257

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-86)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12144.36 Da
• Isoelectric Point: 9.7878

>T76339 WP_011212715.1 NZ_CP021256:68475-68798 [Legionella pneumophila]
MSWIVEFYNESVEEAILTMPPKIQARMIKLLELIETHGANLGPPHTEAMGDGLFEIRAKAQEGIGRSLYCYMKGKHIVVL
HAFVKKSAKTPKPDLQLALKRKREVEK

Nucleotide

• Length: 324 bp

>T76339 NZ_CP021256:68475-68798 [Legionella pneumophila]
ATGTCTTGGATTGTTGAATTTTATAATGAGTCTGTTGAAGAGGCCATACTTACAATGCCGCCAAAAATTCAGGCTCGAAT
GATTAAACTTTTGGAATTGATTGAAACACATGGCGCTAATCTTGGGCCTCCTCATACAGAAGCGATGGGTGATGGGTTAT
TTGAGATTCGCGCAAAAGCTCAAGAAGGAATCGGGCGATCGCTTTATTGCTATATGAAGGGAAAGCATATTGTGGTTTTG
CATGCATTTGTAAAGAAAAGTGCAAAAACTCCAAAACCAGATTTGCAATTAGCTCTCAAAAGAAAGCGGGAGGTAGAAAA
ATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.22 Da
• Isoelectric Point: 9.8787

>AT76339 WP_011212716.1 NZ_CP021256:68795-69079 [Legionella pneumophila]
MMKLKTLKAKALKNPEVKEAYDALESEFALIDALLTMRQKAGLTQEELAERMGTKKGNISRLEKGNTNPSWNTLEKYAHA
CGFDISMAVKRIAS

Nucleotide

• Length: 285 bp

>AT76339 NZ_CP021256:68795-69079 [Legionella pneumophila]
ATGATGAAACTTAAAACATTAAAGGCTAAAGCGTTAAAAAATCCTGAAGTAAAAGAAGCATACGATGCTTTGGAATCCGA
ATTTGCATTGATCGATGCGTTACTGACGATGCGTCAAAAAGCTGGATTAACTCAAGAAGAGTTAGCTGAGCGAATGGGGA
CAAAAAAAGGGAATATTTCTCGACTGGAAAAAGGAAATACTAATCCAAGCTGGAATACTTTAGAGAAATATGCACATGCG
TGTGGGTTTGATATTTCAATGGCAGTAAAGAGAATCGCTTCATAA

Structures

Toxin

• AlphaFold DB: A0A128WF47