Detailed information of TA system TA076336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1711658..1712238
• Replicon: chromosome
• Accession: NZ_CP021248
• Organism: Novosphingobium sp. KA1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CA833_RS25110
• Protein ID: WP_207080593.1
• Coordinates: 1711658..1711939 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: CA833_RS25115
• Protein ID: WP_207081329.1
• Coordinates: 1711957..1712238 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA833_RS25095	1706751..1708088	+	1338	WP_207080590.1	dGTPase	-
CA833_RS25100	1708146..1708718	-	573	WP_207080591.1	hypothetical protein	-
CA833_RS25105	1709380..1710918	-	1539	WP_207080592.1	DUF1593 domain-containing protein	-
CA833_RS25110	1711658..1711939	+	282	WP_207080593.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CA833_RS25115	1711957..1712238	+	282	WP_207081329.1	HigA family addiction module antidote protein	Antitoxin
CA833_RS25120	1712689..1712901	-	213	Protein_1447	transposase	-
CA833_RS25125	1713827..1715773	+	1947	WP_207080594.1	hypothetical protein	-
CA833_RS25130	1715981..1716658	-	678	Protein_1449	Fic family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-66)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10533.96 Da
• Isoelectric Point: 5.1116

>T76336 WP_207080593.1 NZ_CP021248:1711658-1711939 [Novosphingobium sp. KA1]
MIQSAKGKLVEQVLAGKPEKGFPNDIFRAALRKIAMLEAAVNLDDLRSPPGNRLEALSDDREGQHSIRINDQWRICFVWT
DSGPQDVEIMDYH

Nucleotide

• Length: 282 bp

>T76336 NZ_CP021248:1711658-1711939 [Novosphingobium sp. KA1]
ATGATACAGAGCGCCAAGGGCAAGTTGGTTGAACAAGTCTTGGCGGGCAAGCCGGAAAAGGGCTTCCCGAATGACATCTT
CCGGGCCGCTTTGCGGAAAATAGCCATGCTTGAGGCAGCGGTAAATCTGGACGATCTGCGGTCGCCTCCAGGCAACAGGC
TAGAAGCCCTGTCCGATGACCGTGAGGGCCAGCATTCGATCCGTATCAACGACCAATGGCGTATCTGTTTCGTCTGGACC
GACAGCGGCCCGCAAGACGTAGAGATCATGGACTATCATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10537.24 Da
• Isoelectric Point: 6.6405

>AT76336 WP_207081329.1 NZ_CP021248:1711957-1712238 [Novosphingobium sp. KA1]
MLKTPVHPGEILKHEFLEEMGISAGKLAKHIHVPRTRIERLCEERTSVTPDTAKRLSKALGTTPEFWLNLQTHYDLLVDQ
AADIEEIKPLLVA

Nucleotide

• Length: 282 bp

>AT76336 NZ_CP021248:1711957-1712238 [Novosphingobium sp. KA1]
ATGTTGAAAACCCCGGTCCATCCCGGCGAAATCCTCAAGCATGAGTTCCTTGAGGAGATGGGCATTTCTGCTGGCAAGCT
GGCGAAGCATATCCATGTTCCTCGCACCCGCATCGAACGACTGTGCGAGGAGAGGACTTCGGTCACGCCGGACACTGCCA
AGCGTCTATCCAAGGCACTTGGTACGACGCCGGAGTTCTGGCTTAACCTCCAGACGCATTATGATTTGCTGGTGGATCAG
GCAGCGGACATTGAGGAAATCAAGCCGTTGCTGGTGGCTTGA