Detailed information of TA system TA076323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 10863..11481
• Replicon: chromosome
• Accession: NZ_CP021219
• Organism: Sinorhizobium meliloti RU11/001

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q92R61
• Locus tag: SMRU11_RS19205
• Protein ID: WP_010969011.1
• Coordinates: 10863..11159 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SMRU11_RS19210
• Protein ID: WP_010969010.1
• Coordinates: 11173..11481 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMRU11_RS19180	6176..6664	+	489	WP_013844230.1	BA14K family protein	-
SMRU11_RS19185	6878..7927	+	1050	WP_013844229.1	porin	-
SMRU11_RS19190	7968..9035	+	1068	WP_017265823.1	site-specific integrase	-
SMRU11_RS19195	9145..10182	-	1038	WP_003527304.1	porin	-
SMRU11_RS19200	10510..10815	+	306	WP_003527305.1	hypothetical protein	-
SMRU11_RS19205	10863..11159	+	297	WP_010969011.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SMRU11_RS19210	11173..11481	+	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
SMRU11_RS19215	11471..11722	+	252	WP_042708639.1	hypothetical protein	-
SMRU11_RS19225	12151..13272	+	1122	WP_010969009.1	pyridoxal phosphate-dependent aminotransferase	-
SMRU11_RS19230	13294..13560	-	267	WP_003527312.1	sel1 repeat family protein	-
SMRU11_RS19235	14145..14486	+	342	WP_010969008.1	DUF2147 domain-containing protein	-
SMRU11_RS19240	14710..15411	-	702	WP_014529127.1	glutathione S-transferase N-terminal domain-containing protein	-
SMRU11_RS19245	15647..15820	+	174	WP_003527318.1	hypothetical protein	-
SMRU11_RS19250	15834..16007	+	174	WP_010969006.1	hypothetical protein	-
SMRU11_RS19255	16082..16402	+	321	WP_003527320.1	YnfA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.11 Da
• Isoelectric Point: 6.7260

>T76323 WP_010969011.1 NZ_CP021219:10863-11159 [Sinorhizobium meliloti RU11/001]
VIVGFRDDWLRTFFVDDVRSRNIPYDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T76323 NZ_CP021219:10863-11159 [Sinorhizobium meliloti RU11/001]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTACGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT76323 WP_010969010.1 NZ_CP021219:11173-11481 [Sinorhizobium meliloti RU11/001]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT76323 NZ_CP021219:11173-11481 [Sinorhizobium meliloti RU11/001]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: Q92R61