Detailed information of TA system TA076309

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 20379..20805
• Replicon: plasmid p2474-NDM1
• Accession: NZ_CP021210
• Organism: Escherichia coli strain strain Z247

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CA270_RS27775
• Protein ID: WP_001312861.1
• Coordinates: 20379..20537 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	20581..20805 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA270_RS27730	15402..15629	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
CA270_RS27735	15766..16437	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
CA270_RS27740	16631..17014	-	384	WP_001354030.1	relaxosome protein TraM	-
CA270_RS27745	17337..17939	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
CA270_RS27750	18236..19057	-	822	WP_001234445.1	DUF945 domain-containing protein	-
CA270_RS27755	19168..19464	-	297	WP_001272251.1	hypothetical protein	-
CA270_RS28930	19764..20141	+	378	Protein_28	hypothetical protein	-
CA270_RS27775	20379..20537	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	20581..20805	-	225	NuclAT_0	-	Antitoxin
-	20581..20805	-	225	NuclAT_0	-	Antitoxin
-	20581..20805	-	225	NuclAT_0	-	Antitoxin
-	20581..20805	-	225	NuclAT_0	-	Antitoxin
CA270_RS28860	20617..20796	+	180	WP_001309233.1	hypothetical protein	-
CA270_RS27785	20817..21536	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
CA270_RS27790	21533..21967	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
CA270_RS27795	22036..24059	-	2024	Protein_33	ParB/RepB/Spo0J family partition protein	-
CA270_RS27800	24120..24353	-	234	WP_000006003.1	DUF905 family protein	-
CA270_RS27805	24411..24938	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaNDM-1 / aph(3')-VI	-	1..75553	75553

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T76309 WP_001312861.1 NZ_CP021210:c20537-20379 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T76309 NZ_CP021210:c20537-20379 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT76309 NZ_CP021210:c20805-20581 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin