Detailed information of TA system TA076295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2394038..2394837
• Replicon: chromosome
• Accession: NZ_CP021207
• Organism: Escherichia coli strain strain Z247

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: CA270_RS12320
• Protein ID: WP_061089163.1
• Coordinates: 2394373..2394837 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: CA270_RS12315
• Protein ID: WP_001296435.1
• Coordinates: 2394038..2394373 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA270_RS12300	2389823..2390593	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
CA270_RS12305	2390609..2391943	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
CA270_RS12310	2392318..2393889	+	1572	WP_024008768.1	galactarate dehydratase	-
CA270_RS12315	2394038..2394373	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
CA270_RS12320	2394373..2394837	+	465	WP_061089163.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
CA270_RS12325	2394892..2395701	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
CA270_RS12335	2395950..2397230	+	1281	WP_000681950.1	tagatose-bisphosphate aldolase subunit KbaZ	-
CA270_RS12340	2397253..2397726	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
CA270_RS12345	2397737..2398516	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
CA270_RS12350	2398506..2399384	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
CA270_RS12355	2399402..2399836	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17749.09 Da
• Isoelectric Point: 9.4947

>T76295 WP_061089163.1 NZ_CP021207:2394373-2394837 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVAHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T76295 NZ_CP021207:2394373-2394837 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGCCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT76295 WP_001296435.1 NZ_CP021207:2394038-2394373 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT76295 NZ_CP021207:2394038-2394373 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: S1PPV5