Detailed information of TA system TA076277

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 442271..442642
• Replicon: chromosome
• Accession: NZ_CP021207
• Organism: Escherichia coli strain strain Z247

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: CA270_RS02340
• Protein ID: WP_001317028.1
• Coordinates: 442448..442642 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	442271..442449 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA270_RS02315	438227..439600	+	1374	WP_000123789.1	ATP-dependent RNA helicase DbpA	-
CA270_RS02320	439728..440663	-	936	WP_001157412.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
CA270_RS02325	440715..441950	-	1236	WP_000040852.1	site-specific integrase	-
CA270_RS02330	441952..442167	-	216	WP_000079604.1	excisionase XisR	-
CA270_RS02335	442246..442455	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	442271..442449	+	179	NuclAT_0	-	Antitoxin
-	442271..442449	+	179	NuclAT_0	-	Antitoxin
-	442271..442449	+	179	NuclAT_0	-	Antitoxin
-	442271..442449	+	179	NuclAT_0	-	Antitoxin
CA270_RS02340	442448..442642	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
CA270_RS02345	442699..443508	-	810	WP_000166319.1	recombination protein RecT	-
CA270_RS02350	443501..446101	-	2601	WP_086353125.1	exodeoxyribonuclease VIII	-
CA270_RS02355	446203..446478	-	276	WP_000632297.1	protein RacC	-
CA270_RS02360	446553..446723	-	171	WP_001352098.1	conserved protein, Rac prophage	-
CA270_RS02365	446723..446944	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	440715..487732	47017

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T76277 WP_001317028.1 NZ_CP021207:c442642-442448 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T76277 NZ_CP021207:c442642-442448 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT76277 NZ_CP021207:442271-442449 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin