Detailed information of TA system TA076272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 79715..80372
• Replicon: plasmid p1002-NDM1
• Accession: NZ_CP021206
• Organism: Escherichia coli strain Z1002

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: CA268_RS29530
• Protein ID: WP_000270043.1
• Coordinates: 79715..80065 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CA268_RS29535
• Protein ID: WP_000124640.1
• Coordinates: 80070..80372 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA268_RS29490	76189..76686	-	498	WP_000062185.1	hypothetical protein	-
CA268_RS29495	76689..77177	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
CA268_RS29500	77274..77609	+	336	WP_000683477.1	hypothetical protein	-
CA268_RS29505	77624..78094	-	471	WP_001281821.1	hypothetical protein	-
CA268_RS29510	78087..78458	-	372	WP_000516918.1	hypothetical protein	-
CA268_RS29515	78469..78663	-	195	WP_000343597.1	hypothetical protein	-
CA268_RS29525	79004..79552	-	549	WP_001061573.1	hypothetical protein	-
CA268_RS29530	79715..80065	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CA268_RS29535	80070..80372	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
CA268_RS29540	80399..80692	-	294	WP_001239998.1	hypothetical protein	-
CA268_RS29545	80781..81053	-	273	WP_001043046.1	HU family DNA-binding protein	-
CA268_RS29550	81111..81638	-	528	WP_004201083.1	thermonuclease family protein	-
CA268_RS30765	81641..81946	-	306	WP_171820680.1	hypothetical protein	-
CA268_RS29560	81858..82715	-	858	WP_001167036.1	hypothetical protein	-
CA268_RS29565	82702..82932	-	231	WP_000972665.1	hypothetical protein	-
CA268_RS29570	82932..83450	-	519	WP_000210757.1	nitrite reductase	-
CA268_RS29575	83447..83893	-	447	WP_000919343.1	hypothetical protein	-
CA268_RS29580	83893..84252	-	360	WP_000422769.1	hypothetical protein	-
CA268_RS29585	84310..84738	-	429	WP_000591076.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaNDM-1 / rmtC / sul1 / qacE / mph(A) / aac(3)-IId / blaTEM-1B / blaCTX-M-14	htpB	1..111688	111688

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T76272 WP_000270043.1 NZ_CP021206:79715-80065 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T76272 NZ_CP021206:79715-80065 [Escherichia coli]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT76272 WP_000124640.1 NZ_CP021206:80070-80372 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT76272 NZ_CP021206:80070-80372 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9