Detailed information of TA system TA076265

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 26827..27248
• Replicon: plasmid p1002-4
• Accession: NZ_CP021204
• Organism: Escherichia coli strain Z1002

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: CA268_RS28080
• Protein ID: WP_001302184.1
• Coordinates: 27090..27248 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	26827..27025 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA268_RS28040	22523..23917	+	1395	WP_001037799.1	carbohydrate porin	-
CA268_RS28045	24163..24267	-	105	Protein_25	thiosulfate reductase cytochrome B subunit	-
CA268_RS28055	24324..25017	+	694	Protein_26	IS1 family transposase	-
CA268_RS28060	25033..25656	+	624	WP_000912556.1	potassium channel family protein	-
CA268_RS28065	25741..25992	+	252	Protein_28	iron-containing alcohol dehydrogenase	-
CA268_RS28070	26281..26598	-	318	Protein_29	IS110 family transposase	-
CA268_RS28075	26753..26858	+	106	Protein_30	plasmid SOS inhibition protein A	-
-	26827..27025	+	199	NuclAT_0	-	Antitoxin
-	26827..27025	+	199	NuclAT_0	-	Antitoxin
-	26827..27025	+	199	NuclAT_0	-	Antitoxin
-	26827..27025	+	199	NuclAT_0	-	Antitoxin
CA268_RS28080	27090..27248	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
CA268_RS30820	27621..27914	+	294	WP_175271036.1	hypothetical protein	-
CA268_RS30725	27916..28122	+	207	WP_000275854.1	hypothetical protein	-
CA268_RS28095	28147..28434	+	288	WP_000107242.1	hypothetical protein	-
CA268_RS28100	28553..29374	+	822	WP_001234465.1	DUF945 domain-containing protein	-
CA268_RS28105	29670..30242	-	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
CA268_RS28110	30612..30995	+	384	WP_001063020.1	relaxosome protein TraM	-
CA268_RS28115	31186..31833	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
CA268_RS28120	31969..32184	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..92438	92438

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T76265 WP_001302184.1 NZ_CP021204:27090-27248 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T76265 NZ_CP021204:27090-27248 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT76265 NZ_CP021204:26827-27025 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin