Detailed information of TA system TA076261

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 134787..135057
• Replicon: plasmid p1002-1
• Accession: NZ_CP021203
• Organism: Escherichia coli strain Z1002

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CA268_RS27560
• Protein ID: WP_001312861.1
• Coordinates: 134899..135057 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	134787..134850 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA268_RS27530	130498..131025	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
CA268_RS27535	131083..131316	+	234	WP_000006003.1	DUF905 family protein	-
CA268_RS27540	131377..133400	+	2024	Protein_148	ParB/RepB/Spo0J family partition protein	-
CA268_RS27545	133469..133903	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
CA268_RS27550	133900..134619	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	134631..134855	+	225	NuclAT_0	-	-
-	134631..134855	+	225	NuclAT_0	-	-
-	134631..134855	+	225	NuclAT_0	-	-
-	134631..134855	+	225	NuclAT_0	-	-
CA268_RS30705	134640..134819	-	180	WP_001309233.1	hypothetical protein	-
-	134787..134850	-	64	-	-	Antitoxin
CA268_RS27560	134899..135057	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CA268_RS30810	135295..135672	-	378	Protein_153	hypothetical protein	-
CA268_RS27580	135972..136268	+	297	WP_001272251.1	hypothetical protein	-
CA268_RS27585	136379..137200	+	822	WP_001234445.1	DUF945 domain-containing protein	-
CA268_RS27590	137497..138099	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
CA268_RS27595	138422..138805	+	384	WP_001354030.1	relaxosome protein TraM	-
CA268_RS27600	138999..139670	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
CA268_RS27605	139807..140034	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qacE / sul1 / mph(A) / erm(B) / aac(3)-IIa / blaCTX-M-15 / aadA5	-	1..183508	183508
	-	inside	Integron	qacE / aadA5 / dfrA17	-	37..183442	183405

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T76261 WP_001312861.1 NZ_CP021203:134899-135057 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T76261 NZ_CP021203:134899-135057 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT76261 NZ_CP021203:c134850-134787 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin