Detailed information of TA system TA076255

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4532841..4533568
• Replicon: chromosome
• Accession: NZ_CP021202
• Organism: Escherichia coli strain Z1002

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: CA268_RS23370
• Protein ID: WP_001521139.1
• Coordinates: 4533257..4533568 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CA268_RS23365
• Protein ID: WP_000126294.1
• Coordinates: 4532841..4533260 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA268_RS23345	4527926..4528453	-	528	WP_001078777.1	electron transport protein HydN	-
CA268_RS23350	4528602..4529612	-	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
CA268_RS23355	4529872..4531329	+	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
CA268_RS23360	4531338..4532762	+	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
CA268_RS23365	4532841..4533260	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
CA268_RS23370	4533257..4533568	-	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
CA268_RS23375	4533735..4534205	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
CA268_RS23380	4534198..4534608	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
CA268_RS23385	4534605..4535372	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
CA268_RS23390	4535372..4535914	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
CA268_RS23395	4535924..4537633	-	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T76255 WP_001521139.1 NZ_CP021202:c4533568-4533257 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T76255 NZ_CP021202:c4533568-4533257 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTATACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT76255 WP_000126294.1 NZ_CP021202:c4533260-4532841 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT76255 NZ_CP021202:c4533260-4532841 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCCGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTACGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGTGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A829L2T9