Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3713435..3713937 Replicon chromosome
Accession NZ_CP021202
Organism Escherichia coli strain Z1002

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CA268_RS19315 Protein ID WP_000767829.1
Coordinates 3713435..3713689 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CA268_RS19320 Protein ID WP_001259255.1
Coordinates 3713686..3713937 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA268_RS19290 3708450..3708677 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CA268_RS19295 3708691..3709749 - 1059 WP_000492324.1 transport protein YeeE -
CA268_RS19300 3709928..3711286 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CA268_RS30220 3711276..3711338 - 63 WP_010723108.1 membrane protein YoeI -
CA268_RS19305 3711553..3712482 - 930 WP_023154044.1 LysR family transcriptional regulator -
CA268_RS19310 3712528..3713352 - 825 WP_000754758.1 SDR family oxidoreductase -
CA268_RS19315 3713435..3713689 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CA268_RS19320 3713686..3713937 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CA268_RS19325 3714219..3714269 + 51 WP_001364200.1 his operon leader peptide -
CA268_RS19335 3714415..3715314 + 900 WP_021523143.1 ATP phosphoribosyltransferase -
CA268_RS19340 3715320..3716624 + 1305 WP_021523144.1 histidinol dehydrogenase -
CA268_RS19345 3716621..3717691 + 1071 WP_000108937.1 histidinol-phosphate transaminase -
CA268_RS19350 3717691..3718758 + 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T76254 WP_000767829.1 NZ_CP021202:c3713689-3713435 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T76254 NZ_CP021202:c3713689-3713435 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT76254 WP_001259255.1 NZ_CP021202:c3713937-3713686 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT76254 NZ_CP021202:c3713937-3713686 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References