Detailed information of TA system TA076232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1701390..1702084
• Replicon: chromosome
• Accession: NZ_CP021202
• Organism: Escherichia coli strain Z1002

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: CA268_RS08715
• Protein ID: WP_001521903.1
• Coordinates: 1701686..1702084 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: CA268_RS08710
• Protein ID: WP_000554758.1
• Coordinates: 1701390..1701683 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA268_RS08685	1696505..1697254	+	750	WP_016233951.1	C40 family peptidase	-
CA268_RS08690	1697311..1697664	-	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
CA268_RS08695	1697743..1699455	-	1713	Protein_1548	flagellar biosynthesis protein FlhA	-
CA268_RS08700	1699427..1700212	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
CA268_RS08705	1700283..1701338	+	1056	WP_001226177.1	DNA polymerase IV	-
CA268_RS08710	1701390..1701683	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CA268_RS08715	1701686..1702084	+	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CA268_RS08720	1702094..1702546	+	453	WP_023144376.1	GNAT family N-acetyltransferase	-
CA268_RS08725	1702791..1702997	+	207	Protein_1554	RtcB family protein	-
CA268_RS30830	1702993..1703345	+	353	Protein_1555	peptide chain release factor H	-
CA268_RS08735	1703402..1704859	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
CA268_RS08740	1705120..1705578	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CA268_RS08745	1705670..1706914	+	1245	WP_000189541.1	esterase FrsA	-
-	1706174..1706254	+	81	NuclAT_11	-	-
-	1706174..1706254	+	81	NuclAT_11	-	-
-	1706174..1706254	+	81	NuclAT_11	-	-
-	1706174..1706254	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T76232 WP_001521903.1 NZ_CP021202:1701686-1702084 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T76232 NZ_CP021202:1701686-1702084 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT76232 WP_000554758.1 NZ_CP021202:1701390-1701683 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT76232 NZ_CP021202:1701390-1701683 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3