Detailed information of TA system TA076225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 733999..734601
• Replicon: chromosome
• Accession: NZ_CP021202
• Organism: Escherichia coli strain Z1002

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CA268_RS03750
• Protein ID: WP_000897305.1
• Coordinates: 733999..734310 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CA268_RS03755
• Protein ID: WP_000356395.1
• Coordinates: 734311..734601 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA268_RS03725	729932..730390	+	459	WP_000526135.1	IS200/IS605 family transposase	-
CA268_RS03735	730879..732693	+	1815	WP_020233236.1	hypothetical protein	-
CA268_RS03745	732862..733770	-	909	WP_001306651.1	alpha/beta hydrolase	-
CA268_RS03750	733999..734310	+	312	WP_000897305.1	hypothetical protein	Toxin
CA268_RS03755	734311..734601	+	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
CA268_RS03760	734686..734898	+	213	WP_000197769.1	hypothetical protein	-
CA268_RS03765	734960..735238	+	279	WP_001306650.1	hypothetical protein	-
CA268_RS03770	735657..735875	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CA268_RS03775	736094..736336	+	243	WP_021523315.1	hypothetical protein	-
CA268_RS03780	736518..737447	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CA268_RS03785	737444..738079	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CA268_RS03790	738076..738978	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	729932..730390	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T76225 WP_000897305.1 NZ_CP021202:733999-734310 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T76225 NZ_CP021202:733999-734310 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT76225 WP_000356395.1 NZ_CP021202:734311-734601 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT76225 NZ_CP021202:734311-734601 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7