Detailed information of TA system TA076217

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 91594..91836
• Replicon: plasmid pEC-81009
• Accession: NZ_CP021180
• Organism: Escherichia coli strain 81009

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: DNNLJILF_RS26535
• Protein ID: WP_001312861.1
• Coordinates: 91678..91836 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	91594..91634 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DNNLJILF_RS26510	86789..88747	+	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
DNNLJILF_RS26515	88802..89236	+	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
DNNLJILF_RS26520	89233..89952	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
-	89964..90150	+	187	NuclAT_0	-	-
-	89964..90150	+	187	NuclAT_0	-	-
-	89964..90150	+	187	NuclAT_0	-	-
-	89964..90150	+	187	NuclAT_0	-	-
DNNLJILF_RS26530	90198..91567	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	91594..91634	+	41	NuclAT_1	-	Antitoxin
-	91594..91634	+	41	NuclAT_1	-	Antitoxin
-	91594..91634	+	41	NuclAT_1	-	Antitoxin
-	91594..91634	+	41	NuclAT_1	-	Antitoxin
DNNLJILF_RS26535	91678..91836	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
DNNLJILF_RS27865	92279..92614	+	336	WP_013023876.1	hypothetical protein	-
DNNLJILF_RS26550	92527..92733	+	207	WP_000275859.1	hypothetical protein	-
DNNLJILF_RS26555	92758..93045	+	288	WP_000107535.1	hypothetical protein	-
DNNLJILF_RS26560	93164..93985	+	822	WP_001234469.1	DUF945 domain-containing protein	-
DNNLJILF_RS26565	94282..94884	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
DNNLJILF_RS26570	95215..95598	+	384	WP_001151566.1	relaxosome protein TraM	-
DNNLJILF_RS26575	95732..96409	+	678	WP_001348626.1	PAS domain-containing protein	-
DNNLJILF_RS26580	96497..96724	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA17 / aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27	senB	1..135720	135720

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T76217 WP_001312861.1 NZ_CP021180:91678-91836 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T76217 NZ_CP021180:91678-91836 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT76217 NZ_CP021180:91594-91634 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin