Detailed information of TA system TA076190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 366464..367066
• Replicon: chromosome
• Accession: NZ_CP021179
• Organism: Escherichia coli strain 81009

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: DNNLJILF_RS01815
• Protein ID: WP_000897302.1
• Coordinates: 366755..367066 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DNNLJILF_RS01810
• Protein ID: WP_000356397.1
• Coordinates: 366464..366754 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DNNLJILF_RS01770	361771..362700	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
DNNLJILF_RS01775	362882..363124	-	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
DNNLJILF_RS01780	363414..364262	+	849	WP_001038650.1	hypothetical protein	-
DNNLJILF_RS01785	364578..365027	+	450	WP_032140890.1	hypothetical protein	-
DNNLJILF_RS01790	365212..365430	-	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
DNNLJILF_RS01800	365827..366105	-	279	WP_001296612.1	hypothetical protein	-
DNNLJILF_RS01810	366464..366754	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
DNNLJILF_RS01815	366755..367066	-	312	WP_000897302.1	hypothetical protein	Toxin
DNNLJILF_RS01820	367295..368203	+	909	WP_001385591.1	alpha/beta hydrolase	-
DNNLJILF_RS01825	368267..369208	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
DNNLJILF_RS01830	369253..369690	-	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
DNNLJILF_RS01835	369687..370559	-	873	WP_000920762.1	virulence factor BrkB family protein	-
DNNLJILF_RS01840	370553..371152	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T76190 WP_000897302.1 NZ_CP021179:c367066-366755 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T76190 NZ_CP021179:c367066-366755 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT76190 WP_000356397.1 NZ_CP021179:c366754-366464 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT76190 NZ_CP021179:c366754-366464 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416