Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3389377..3389879 Replicon chromosome
Accession NZ_CP021175
Organism Escherichia coli strain 5CRE51

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CA593_RS17365 Protein ID WP_000767829.1
Coordinates 3389625..3389879 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CA593_RS17360 Protein ID WP_001259255.1
Coordinates 3389377..3389628 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA593_RS17330 3384554..3385621 - 1068 WP_000080055.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CA593_RS17335 3385621..3386691 - 1071 WP_000108933.1 histidinol-phosphate transaminase -
CA593_RS17340 3386688..3387992 - 1305 WP_001352244.1 histidinol dehydrogenase -
CA593_RS17345 3387998..3388897 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CA593_RS17355 3389043..3389093 - 51 WP_001364200.1 his operon leader peptide -
CA593_RS17360 3389377..3389628 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CA593_RS17365 3389625..3389879 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CA593_RS17370 3389962..3390786 + 825 WP_000754737.1 SDR family oxidoreductase -
CA593_RS17375 3390832..3391761 + 930 WP_000803366.1 LysR family transcriptional regulator -
CA593_RS27775 3391976..3392038 + 63 WP_010723108.1 membrane protein YoeI -
CA593_RS17380 3392028..3393386 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CA593_RS17385 3393565..3394623 + 1059 WP_000492346.1 transport protein YeeE -
CA593_RS17390 3394637..3394864 + 228 WP_000985272.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T76162 WP_000767829.1 NZ_CP021175:3389625-3389879 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T76162 NZ_CP021175:3389625-3389879 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT76162 WP_001259255.1 NZ_CP021175:3389377-3389628 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT76162 NZ_CP021175:3389377-3389628 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References