76162

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3389377..3389879 Replicon chromosome
Accession NZ_CP021175
Organism Escherichia coli strain 5CRE51

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CA593_RS17365 Protein ID WP_000767829.1
Coordinates 3389625..3389879 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CA593_RS17360 Protein ID WP_001259255.1
Coordinates 3389377..3389628 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA593_RS17330 3384554..3385621 - 1068 WP_000080055.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CA593_RS17335 3385621..3386691 - 1071 WP_000108933.1 histidinol-phosphate transaminase -
CA593_RS17340 3386688..3387992 - 1305 WP_001352244.1 histidinol dehydrogenase -
CA593_RS17345 3387998..3388897 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CA593_RS17355 3389043..3389093 - 51 WP_001364200.1 his operon leader peptide -
CA593_RS17360 3389377..3389628 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CA593_RS17365 3389625..3389879 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CA593_RS17370 3389962..3390786 + 825 WP_000754737.1 SDR family oxidoreductase -
CA593_RS17375 3390832..3391761 + 930 WP_000803366.1 LysR family transcriptional regulator -
CA593_RS27775 3391976..3392038 + 63 WP_010723108.1 membrane protein YoeI -
CA593_RS17380 3392028..3393386 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CA593_RS17385 3393565..3394623 + 1059 WP_000492346.1 transport protein YeeE -
CA593_RS17390 3394637..3394864 + 228 WP_000985272.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T76162 WP_000767829.1 NZ_CP021175:3389625-3389879 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T76162 NZ_CP021175:3389625-3389879 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT76162 WP_001259255.1 NZ_CP021175:3389377-3389628 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT76162 NZ_CP021175:3389377-3389628 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References