Detailed information of TA system TA076161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2596278..2597005
• Replicon: chromosome
• Accession: NZ_CP021175
• Organism: Escherichia coli strain 5CRE51

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: CA593_RS13400
• Protein ID: WP_001521139.1
• Coordinates: 2596278..2596589 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CA593_RS13405
• Protein ID: WP_000126294.1
• Coordinates: 2596586..2597005 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA593_RS13375	2592213..2593922	+	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-
CA593_RS13380	2593932..2594474	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
CA593_RS13385	2594474..2595241	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
CA593_RS13390	2595238..2595648	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
CA593_RS13395	2595641..2596111	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
CA593_RS13400	2596278..2596589	+	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
CA593_RS13405	2596586..2597005	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
CA593_RS13410	2597084..2598508	-	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
CA593_RS13415	2598517..2599974	-	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
CA593_RS13420	2600234..2601244	+	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
CA593_RS13425	2601393..2601920	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T76161 WP_001521139.1 NZ_CP021175:2596278-2596589 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T76161 NZ_CP021175:2596278-2596589 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTATACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT76161 WP_000126294.1 NZ_CP021175:2596586-2597005 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT76161 NZ_CP021175:2596586-2597005 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCCGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTACGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGTGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A829L2T9