Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 453578..454272 Replicon chromosome
Accession NZ_CP021175
Organism Escherichia coli strain 5CRE51

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag CA593_RS02435 Protein ID WP_001263489.1
Coordinates 453578..453976 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag CA593_RS02440 Protein ID WP_000554758.1
Coordinates 453979..454272 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 449166..449246 - 81 NuclAT_12 - -
- 449166..449246 - 81 NuclAT_12 - -
- 449166..449246 - 81 NuclAT_12 - -
- 449166..449246 - 81 NuclAT_12 - -
CA593_RS02405 449842..450300 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
CA593_RS02410 450561..452018 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
CA593_RS02415 452075..452596 - 522 Protein_433 peptide chain release factor H -
CA593_RS02420 452592..452798 - 207 Protein_434 RtcB family protein -
CA593_RS02430 453116..453568 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
CA593_RS02435 453578..453976 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
CA593_RS02440 453979..454272 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
CA593_RS02445 454324..455379 - 1056 WP_001226164.1 DNA polymerase IV -
CA593_RS02450 455450..456235 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
CA593_RS02455 456207..457919 + 1713 Protein_440 flagellar biosynthesis protein FlhA -
CA593_RS02460 458143..458640 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 397633..459442 61809


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T76152 WP_001263489.1 NZ_CP021175:c453976-453578 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T76152 NZ_CP021175:c453976-453578 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT76152 WP_000554758.1 NZ_CP021175:c454272-453979 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT76152 NZ_CP021175:c454272-453979 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References