Detailed information of TA system TA076110

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 31734..32464
• Replicon: chromosome
• Accession: NZ_CP021167
• Organism: Enterobacter hormaechei strain 388

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: CAL61_RS00155
• Protein ID: WP_023302825.1
• Coordinates: 31734..32048 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CAL61_RS00160
• Protein ID: WP_127350686.1
• Coordinates: 32045..32464 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAL61_RS00130	27400..28485	+	1086	Protein_25	cellulase family glycosylhydrolase	-
CAL61_RS00135	28391..29575	-	1185	WP_003861070.1	multidrug efflux MFS transporter EmrD	-
CAL61_RS00140	29751..30584	-	834	WP_023323922.1	DMT family transporter	-
CAL61_RS00145	30647..31093	-	447	WP_003861064.1	GNAT family N-acetyltransferase	-
CAL61_RS00150	31158..31247	-	90	WP_017383683.1	type I toxin-antitoxin system toxin TisB	-
CAL61_RS00155	31734..32048	+	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
CAL61_RS00160	32045..32464	+	420	WP_127350686.1	helix-turn-helix domain-containing protein	Antitoxin
CAL61_RS00165	32611..32709	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
CAL61_RS00170	32816..34504	+	1689	WP_003861056.1	acetolactate synthase large subunit	-
CAL61_RS00175	34508..34795	+	288	WP_028014960.1	acetolactate synthase small subunit	-
CAL61_RS00180	34878..35471	+	594	WP_003861054.1	transcriptional regulator UhpA	-
CAL61_RS00185	35468..36973	+	1506	WP_003861052.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-137)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T76110 WP_023302825.1 NZ_CP021167:31734-32048 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T76110 NZ_CP021167:31734-32048 [Enterobacter hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAACACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATCTTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15502.15 Da
• Isoelectric Point: 6.4840

>AT76110 WP_127350686.1 NZ_CP021167:32045-32464 [Enterobacter hormaechei]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLHIVCARISHYEANRPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT76110 NZ_CP021167:32045-32464 [Enterobacter hormaechei]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCACGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCTAATAGCCCTTTGCTGCATATTGTGT
GCGCCAGAATTAGCCATTACGAAGCTAATCGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGA
ATTGCAGTTTTAAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGAT
GGTTTCACGTGTTCTGAATGGTCAGAGACAGCTTACTCTGAACCATATCAAAAAGCTGGCGGCCAGATTTGGGGTATCAC
CTGCGTTATTTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A837FIL2