Detailed information of TA system TA076057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Gp49-HTH_37
• Location: 23859..24310
• Replicon: plasmid pPDD-Na-1-4
• Accession: NZ_CP021156
• Organism: Photobacterium damselae subsp. damselae strain KC-Na-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CAY62_RS21295
• Protein ID: WP_086368793.1
• Coordinates: 23859..24038 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CAY62_RS21300
• Protein ID: WP_086368794.1
• Coordinates: 24035..24310 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAY62_RS21805	19764..19934	-	171	WP_165001294.1	hypothetical protein	-
CAY62_RS21280	21164..21559	+	396	WP_086368791.1	hypothetical protein	-
CAY62_RS21285	21893..22782	-	890	Protein_26	IS630 family transposase	-
CAY62_RS21290	23020..23697	+	678	WP_086368792.1	CPBP family intramembrane metalloprotease	-
CAY62_RS21295	23859..24038	+	180	WP_086368793.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CAY62_RS21300	24035..24310	+	276	WP_086368794.1	helix-turn-helix domain-containing protein	Antitoxin
CAY62_RS21305	24635..25123	+	489	WP_086368795.1	hypothetical protein	-
CAY62_RS21310	25123..25341	+	219	WP_086368796.1	hypothetical protein	-
CAY62_RS21315	25746..26474	+	729	WP_086368797.1	ParA family protein	-
CAY62_RS21320	26509..26730	+	222	WP_036766439.1	hypothetical protein	-
CAY62_RS21325	26848..27834	+	987	WP_086368798.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..45429	45429
	-	flank	IS/Tn	-	-	22201..22794	593

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (2-55)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6825.12 Da
• Isoelectric Point: 10.5907

>T76057 WP_086368793.1 NZ_CP021156:23859-24038 [Photobacterium damselae subsp. damselae]
MGDGLFEIRAKAQEGIGRGLFCYLKGQHVYVLRAFVKKANKTQKKEIELARERMKEVKL

Nucleotide

• Length: 180 bp

>T76057 NZ_CP021156:23859-24038 [Photobacterium damselae subsp. damselae]
ATGGGAGATGGGCTTTTTGAAATAAGAGCTAAAGCTCAAGAAGGTATAGGTAGAGGTTTGTTTTGCTATTTAAAAGGACA
GCACGTTTATGTATTAAGAGCTTTTGTGAAAAAGGCGAATAAGACCCAAAAAAAAGAAATAGAACTTGCTAGAGAGCGAA
TGAAAGAGGTGAAGTTATGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10072.56 Da
• Isoelectric Point: 8.9869

>AT76057 WP_086368794.1 NZ_CP021156:24035-24310 [Photobacterium damselae subsp. damselae]
MNLSALKRKALQNPEVKEAYDDLSIEFELINSLISMREKSGLTQDEVAEKMGTKAPNISRLESGRSNPSLKTLVSYAQAC
GFKLNIGFKHA

Nucleotide

• Length: 276 bp

>AT76057 NZ_CP021156:24035-24310 [Photobacterium damselae subsp. damselae]
ATGAATTTATCTGCATTAAAAAGAAAAGCATTACAGAATCCTGAAGTAAAAGAAGCTTATGACGATCTAAGTATTGAATT
TGAATTGATAAATTCTCTAATTTCAATGAGAGAAAAATCAGGCTTAACTCAAGATGAAGTTGCTGAAAAAATGGGAACAA
AAGCCCCGAATATTTCGCGTTTAGAATCTGGACGATCAAATCCTAGTCTTAAAACTTTAGTGAGTTATGCTCAAGCATGT
GGATTTAAGCTAAATATTGGTTTTAAACATGCTTAA