Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4244592..4245133 Replicon chromosome
Accession NZ_CP021144
Organism Shigella sonnei strain 2015AM-1099

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag B9127_RS23360 Protein ID WP_000615976.1
Coordinates 4244592..4244870 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag B9127_RS23365 Protein ID WP_000729704.1
Coordinates 4244873..4245133 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9127_RS23345 (4242174) 4242174..4242752 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
B9127_RS23350 (4242958) 4242958..4243725 + 768 WP_000333380.1 class II glutamine amidotransferase -
B9127_RS23355 (4243696) 4243696..4244436 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
B9127_RS23360 (4244592) 4244592..4244870 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
B9127_RS23365 (4244873) 4244873..4245133 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
B9127_RS27585 (4246006) 4246006..4246134 + 129 Protein_4188 ISAs1 family transposase -
B9127_RS23380 (4246205) 4246205..4246702 + 498 WP_000006258.1 REP-associated tyrosine transposase RayT -
B9127_RS23385 (4246825) 4246825..4248537 - 1713 Protein_4190 flagellar biosynthesis protein FlhA -
B9127_RS23390 (4248509) 4248509..4249294 + 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4234442..4246702 12260


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T76049 WP_000615976.1 NZ_CP021144:c4244870-4244592 [Shigella sonnei]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T76049 NZ_CP021144:c4244870-4244592 [Shigella sonnei]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT76049 WP_000729704.1 NZ_CP021144:c4245133-4244873 [Shigella sonnei]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT76049 NZ_CP021144:c4245133-4244873 [Shigella sonnei]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCTGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References