Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2401373..2402100 Replicon chromosome
Accession NZ_CP021144
Organism Shigella sonnei strain 2015AM-1099

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag B9127_RS13470 Protein ID WP_000550189.1
Coordinates 2401373..2401687 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag B9127_RS13475 Protein ID WP_000560263.1
Coordinates 2401684..2402100 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9127_RS13445 (2397539) 2397539..2398525 - 987 WP_001446103.1 Gfo/Idh/MocA family oxidoreductase -
B9127_RS13450 (2398604) 2398604..2399287 - 684 WP_001183060.1 vancomycin high temperature exclusion protein -
B9127_RS13455 (2399364) 2399364..2399867 - 504 WP_001305117.1 M48 family metallopeptidase -
B9127_RS13460 (2399952) 2399952..2401088 + 1137 WP_000018685.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
B9127_RS13470 (2401373) 2401373..2401687 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
B9127_RS13475 (2401684) 2401684..2402100 + 417 WP_000560263.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
B9127_RS13480 (2402145) 2402145..2404163 - 2019 WP_000121451.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
B9127_RS13485 (2404389) 2404389..2406740 - 2352 WP_039059561.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T76046 WP_000550189.1 NZ_CP021144:2401373-2401687 [Shigella sonnei]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T76046 NZ_CP021144:2401373-2401687 [Shigella sonnei]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14979.42 Da        Isoelectric Point: 4.4547

>AT76046 WP_000560263.1 NZ_CP021144:2401684-2402100 [Shigella sonnei]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLADLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT76046 NZ_CP021144:2401684-2402100 [Shigella sonnei]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCGGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTGCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References