Detailed information of TA system TA076043

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2124515..2125242
• Replicon: chromosome
• Accession: NZ_CP021144
• Organism: Shigella sonnei strain 2015AM-1099

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: B9127_RS12015
• Protein ID: WP_000547563.1
• Coordinates: 2124931..2125242 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B9127_RS12010
• Protein ID: WP_000126296.1
• Coordinates: 2124515..2124934 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9127_RS11990 (2119566)	2119566..2120093	-	528	WP_001078766.1	electron transport protein HydN	-
B9127_RS11995 (2120241)	2120241..2121251	-	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
B9127_RS12000 (2121511)	2121511..2122968	+	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
B9127_RS12005 (2122977)	2122977..2124401	+	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
B9127_RS12010 (2124515)	2124515..2124934	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
B9127_RS12015 (2124931)	2124931..2125242	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
B9127_RS12020 (2125404)	2125404..2126177	-	774	WP_001026459.1	hypothetical protein	-
B9127_RS12025 (2126202)	2126202..2126672	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
B9127_RS12030 (2126665)	2126665..2127075	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
B9127_RS12035 (2127072)	2127072..2127839	-	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
B9127_RS12040 (2127839)	2127839..2128381	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
B9127_RS12045 (2128391)	2128391..2130088	-	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T76043 WP_000547563.1 NZ_CP021144:c2125242-2124931 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T76043 NZ_CP021144:c2125242-2124931 [Shigella sonnei]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT76043 WP_000126296.1 NZ_CP021144:c2124934-2124515 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT76043 NZ_CP021144:c2124934-2124515 [Shigella sonnei]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22