Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 4477804..4478414 Replicon chromosome
Accession NZ_CP021108
Organism Bordetella genomosp. 8 strain AU19157

Toxin (Protein)


Gene name higB Uniprot ID A0A261RLU1
Locus tag CAL12_RS20320 Protein ID WP_066671154.1
Coordinates 4478103..4478414 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A261RLY7
Locus tag CAL12_RS20315 Protein ID WP_066671150.1
Coordinates 4477804..4478100 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CAL12_RS20305 4472919..4474121 - 1203 WP_198298279.1 hypothetical protein -
CAL12_RS20310 4474099..4476771 - 2673 WP_086066277.1 ATP-binding protein -
CAL12_RS20315 4477804..4478100 - 297 WP_066671150.1 helix-turn-helix domain-containing protein Antitoxin
CAL12_RS20320 4478103..4478414 - 312 WP_066671154.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CAL12_RS20325 4478550..4480520 - 1971 WP_086066278.1 ParB N-terminal domain-containing protein -
CAL12_RS28325 4480550..4480699 - 150 WP_198298280.1 hypothetical protein -
CAL12_RS20330 4480798..4481214 - 417 WP_086066279.1 hypothetical protein -
CAL12_RS20335 4481235..4481648 - 414 WP_086066280.1 single-stranded DNA-binding protein -
CAL12_RS20340 4482653..4482907 - 255 WP_086066281.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 4435873..4538908 103035


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(41-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-96)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11690.54 Da        Isoelectric Point: 5.8806

>T75990 WP_066671154.1 NZ_CP021108:c4478414-4478103 [Bordetella genomosp. 8]
MEFIETPVFTKLITALLSDEEYTGLQKLLIENPERGDLIRGGGGIRKVRYGRQGTGKSGGIRVIYYWLSEDHQIYMLVAY
PKSKKDNLTPDEIAVLCELVKEL

Download         Length: 312 bp

>T75990 NZ_CP021108:c4478414-4478103 [Bordetella genomosp. 8]
ATGGAATTTATCGAGACCCCGGTTTTCACGAAGCTGATTACCGCGTTGTTGTCGGATGAGGAATATACCGGGCTGCAAAA
GCTGCTGATTGAGAACCCCGAGCGAGGCGATCTCATCCGAGGCGGCGGCGGCATACGGAAGGTGCGCTACGGTCGGCAAG
GTACTGGCAAGAGCGGCGGAATTCGGGTCATCTATTACTGGCTCAGCGAAGACCACCAAATTTACATGCTGGTGGCGTAC
CCGAAATCCAAGAAGGACAACCTGACACCTGACGAGATAGCCGTGCTGTGCGAACTGGTCAAGGAGCTGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 10867.54 Da        Isoelectric Point: 10.8660

>AT75990 WP_066671150.1 NZ_CP021108:c4478100-4477804 [Bordetella genomosp. 8]
MKKDDTLFNELQTSLKQAAAIRKGKMQPSRVFKVETVDVKATREKTHLSQTEFAEAIQVSVRTLQNWEQGHRNPTGPAVA
LLKIVASAPDVAVRALRA

Download         Length: 297 bp

>AT75990 NZ_CP021108:c4478100-4477804 [Bordetella genomosp. 8]
ATGAAGAAGGACGACACTCTGTTTAACGAACTGCAGACCAGCCTGAAGCAAGCGGCGGCTATTCGCAAAGGGAAGATGCA
GCCCTCGCGTGTGTTCAAGGTCGAGACGGTGGACGTCAAGGCGACGCGCGAGAAGACGCACCTGTCTCAAACGGAATTCG
CAGAGGCGATTCAAGTCAGCGTCCGCACGCTGCAAAACTGGGAGCAGGGTCATCGCAATCCGACGGGGCCGGCGGTTGCT
TTGCTGAAAATCGTGGCGAGCGCTCCAGATGTCGCCGTGCGTGCGTTGCGGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A261RLU1

Antitoxin

Source ID Structure
AlphaFold DB A0A261RLY7

References