Detailed information of TA system TA075969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1736986..1737500
• Replicon: chromosome
• Accession: NZ_CP021104
• Organism: Limosilactobacillus fermentum strain FTDC 8312

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A6C1EMT8
• Locus tag: B7441_RS08825
• Protein ID: WP_035437199.1
• Coordinates: 1736986..1737249 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: B7441_RS08830
• Protein ID: WP_035437201.1
• Coordinates: 1737246..1737500 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7441_RS08810	1732462..1733244	+	783	WP_035437193.1	dimethylargininase	-
B7441_RS08815	1733632..1735029	+	1398	WP_035437196.1	sodium:proton antiporter	-
B7441_RS08820	1735455..1736576	+	1122	WP_080650628.1	PTS sugar transporter subunit IIC	-
B7441_RS08825	1736986..1737249	-	264	WP_035437199.1	Txe/YoeB family addiction module toxin	Toxin
B7441_RS08830	1737246..1737500	-	255	WP_035437201.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
B7441_RS08835	1737749..1738036	+	288	WP_041807836.1	transposase	-
B7441_RS08840	1738060..1738938	+	879	WP_086031809.1	IS3 family transposase	-
B7441_RS08845	1739736..1740500	+	765	WP_080650602.1	zinc ribbon domain-containing protein	-
B7441_RS08850	1740707..1741612	-	906	WP_154244344.1	IS3 family transposase	-
B7441_RS08855	1741549..1742163	-	615	WP_035431426.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1733543..1796675	63132

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (25-78)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-55)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10252.94 Da
• Isoelectric Point: 10.3349

>T75969 WP_035437199.1 NZ_CP021104:c1737249-1736986 [Limosilactobacillus fermentum]
MSYAIKLKRSANKDLKKIKGSYLEKSFLQIIEQLRKDPLAPNQGFEKLVLPIKGFYSRRINIQHRLVYKVDQDTQTVIIY
SAWSHDE

Nucleotide

• Length: 264 bp

>T75969 NZ_CP021104:c1737249-1736986 [Limosilactobacillus fermentum]
ATGAGTTACGCGATCAAACTCAAGCGGAGCGCTAATAAAGATCTCAAGAAAATTAAGGGGTCATATTTAGAAAAGAGCTT
CTTGCAAATAATTGAGCAGCTCCGTAAAGACCCCTTGGCACCCAACCAAGGCTTTGAAAAATTAGTACTGCCAATTAAGG
GCTTTTACTCCCGGCGGATTAACATTCAGCACCGACTCGTCTATAAGGTTGATCAAGACACGCAAACTGTGATCATCTAT
TCTGCGTGGTCACATGACGAATAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9773.05 Da
• Isoelectric Point: 4.4237

>AT75969 WP_035437201.1 NZ_CP021104:c1737500-1737246 [Limosilactobacillus fermentum]
MDQIVTPTQARKDLFKIIKRINENNEPVVIKPTRADEKSVVVIGEDDWKAIQETLFLVNHGVDKQIKERENEPLDDFDDV
WGQL

Nucleotide

• Length: 255 bp

>AT75969 NZ_CP021104:c1737500-1737246 [Limosilactobacillus fermentum]
ATGGACCAAATTGTAACCCCAACGCAAGCACGGAAAGATTTATTCAAAATAATCAAAAGGATCAATGAAAATAATGAGCC
GGTTGTGATTAAGCCGACCAGGGCGGATGAAAAGAGCGTTGTCGTCATTGGTGAAGATGACTGGAAAGCAATCCAAGAAA
CGTTATTTTTGGTTAATCACGGTGTTGACAAGCAAATTAAAGAACGGGAAAACGAGCCGCTCGACGACTTTGATGATGTT
TGGGGTCAACTATGA

Structures

Toxin

• AlphaFold DB: A0A6C1EMT8