Detailed information of TA system TA075964

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 128181..128607
• Replicon: plasmid p13P460A-2
• Accession: NZ_CP021087
• Organism: Escherichia coli strain 13P460A

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BWI88_RS26135
• Protein ID: WP_001312861.1
• Coordinates: 128181..128339 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	128383..128607 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI88_RS26100	123555..124244	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
BWI88_RS26105	124431..124814	-	384	WP_001151524.1	relaxosome protein TraM	-
BWI88_RS26110	125135..125737	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
BWI88_RS26115	126034..126855	-	822	WP_001234426.1	DUF945 domain-containing protein	-
BWI88_RS26120	126973..127260	-	288	WP_000107535.1	hypothetical protein	-
BWI88_RS26135	128181..128339	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	128383..128607	-	225	NuclAT_0	-	Antitoxin
-	128383..128607	-	225	NuclAT_0	-	Antitoxin
-	128383..128607	-	225	NuclAT_0	-	Antitoxin
-	128383..128607	-	225	NuclAT_0	-	Antitoxin
BWI88_RS26695	128419..128607	+	189	WP_001299721.1	hypothetical protein	-
BWI88_RS26140	128619..129338	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
BWI88_RS26145	129335..129769	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
BWI88_RS26150	129824..130021	-	198	Protein_139	hypothetical protein	-
BWI88_RS26155	130049..130282	-	234	WP_000005987.1	DUF905 family protein	-
BWI88_RS26160	130350..130889	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
BWI88_RS26860	130915..131121	-	207	WP_000275856.1	hypothetical protein	-
BWI88_RS26185	132260..133231	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / tet(B) / catA1 / aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..169505	169505

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T75964 WP_001312861.1 NZ_CP021087:c128339-128181 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T75964 NZ_CP021087:c128339-128181 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT75964 NZ_CP021087:c128607-128383 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin