Detailed information of TA system TA075959

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 89852..90106
• Replicon: plasmid p13P460A-2
• Accession: NZ_CP021087
• Organism: Escherichia coli strain 13P460A

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: BWI88_RS25905
• Protein ID: WP_001351576.1
• Coordinates: 89852..90058 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	90045..90106 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI88_RS25865	85094..85393	-	300	Protein_84	incFII family plasmid replication initiator RepA	-
BWI88_RS25870	85480..86226	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
BWI88_RS25875	86241..87782	-	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
BWI88_RS25880	87974..88537	-	564	Protein_87	incFII family plasmid replication initiator RepA	-
BWI88_RS25885	88530..89012	-	483	WP_001273588.1	hypothetical protein	-
BWI88_RS25890	89005..89079	-	75	WP_001442103.1	RepA leader peptide Tap	-
BWI88_RS25900	89311..89568	-	258	WP_000083845.1	replication regulatory protein RepA	-
BWI88_RS25905	89852..90058	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	90045..90106	+	62	NuclAT_1	-	Antitoxin
-	90045..90106	+	62	NuclAT_1	-	Antitoxin
-	90045..90106	+	62	NuclAT_1	-	Antitoxin
-	90045..90106	+	62	NuclAT_1	-	Antitoxin
BWI88_RS26855	90362..90436	-	75	Protein_92	endonuclease	-
BWI88_RS25915	90682..90894	-	213	WP_001299730.1	ANR family transcriptional regulator	-
BWI88_RS25920	91030..91590	-	561	WP_000139315.1	fertility inhibition protein FinO	-
BWI88_RS25925	91693..92553	-	861	WP_000704514.1	alpha/beta hydrolase	-
BWI88_RS25930	92612..93358	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / tet(B) / catA1 / aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..169505	169505

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T75959 WP_001351576.1 NZ_CP021087:c90058-89852 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T75959 NZ_CP021087:c90058-89852 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT75959 NZ_CP021087:90045-90106 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin