Detailed information of TA system TA075932

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1160103..1160625
• Replicon: chromosome
• Accession: NZ_CP021059
• Organism: Macrococcus canis strain KM 45013

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: MCCS_RS05875
• Protein ID: WP_086042495.1
• Coordinates: 1160103..1160375 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: MCCS_RS05880
• Protein ID: WP_086042496.1
• Coordinates: 1160368..1160625 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCCS_RS05830	1155419..1155805	+	387	WP_086042486.1	sporulation protein	-
MCCS_RS05835	1155868..1156383	-	516	WP_086042487.1	DUF402 domain-containing protein	-
MCCS_RS05840	1156476..1156934	+	459	WP_086042488.1	NUDIX domain-containing protein	-
MCCS_RS05845	1157030..1157281	+	252	WP_086042489.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
MCCS_RS05850	1157281..1157541	+	261	WP_086042490.1	Txe/YoeB family addiction module toxin	-
MCCS_RS05855	1157542..1157991	-	450	WP_086042491.1	GNAT family N-acetyltransferase	-
MCCS_RS05860	1158209..1159099	+	891	WP_086042492.1	phosphotransferase	-
MCCS_RS05865	1159131..1159313	+	183	WP_086042493.1	hypothetical protein	-
MCCS_RS05870	1159319..1160080	+	762	WP_086042494.1	hypothetical protein	-
MCCS_RS05875	1160103..1160375	-	273	WP_086042495.1	Txe/YoeB family addiction module toxin	Toxin
MCCS_RS05880	1160368..1160625	-	258	WP_086042496.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MCCS_RS05885	1160914..1161138	+	225	WP_086042497.1	toxin-antitoxin system, antitoxin component	-
MCCS_RS05890	1161135..1161401	+	267	WP_086042498.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MCCS_RS05895	1161512..1161880	+	369	WP_086042499.1	GntR family transcriptional regulator	-
MCCS_RS05900	1161880..1162980	+	1101	WP_086042500.1	DUF1648 domain-containing protein	-
MCCS_RS05905	1163461..1164723	+	1263	WP_086042501.1	Y-family DNA polymerase	-
MCCS_RS05910	1164876..1165064	+	189	WP_086042502.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10694.45 Da
• Isoelectric Point: 9.7524

>T75932 WP_086042495.1 NZ_CP021059:c1160375-1160103 [Macrococcus canis]
MPKKKILFYSSGFEDYTYWQTNDKTVLKKINKLIKSIMIDGANEGIGKPEKLLGNYTGCYSRRITHEHRLVYKIEDDSIC
ILQCRFHYKR

Nucleotide

• Length: 273 bp

>T75932 NZ_CP021059:c1160375-1160103 [Macrococcus canis]
ATGCCTAAGAAAAAGATACTATTTTATTCAAGTGGTTTTGAAGATTATACGTACTGGCAAACTAACGATAAAACTGTATT
AAAGAAAATAAATAAACTAATAAAGAGTATTATGATTGATGGTGCAAATGAAGGTATAGGTAAGCCAGAAAAATTATTAG
GAAACTATACAGGTTGCTATAGCAGAAGAATCACTCATGAACATAGGCTCGTATATAAGATAGAAGATGATTCAATTTGT
ATTTTACAATGTCGTTTTCATTATAAAAGGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9697.75 Da
• Isoelectric Point: 4.1540

>AT75932 WP_086042496.1 NZ_CP021059:c1160625-1160368 [Macrococcus canis]
MKVTTYSNARSNFRKIIDIVNEDCEPYTITTNTHNAVILSEQDYNSMIETLYLQSTPANAERLQKAIDESTDKNNLLQVE
IDLDA

Nucleotide

• Length: 258 bp

>AT75932 NZ_CP021059:c1160625-1160368 [Macrococcus canis]
ATGAAAGTCACAACTTATTCTAATGCAAGATCAAATTTTAGAAAAATTATAGATATTGTTAATGAAGACTGTGAACCTTA
CACAATCACAACAAATACGCATAATGCCGTGATATTATCTGAACAAGATTATAATAGTATGATTGAAACACTTTATTTAC
AGAGTACACCAGCGAATGCCGAAAGATTACAAAAAGCGATAGATGAATCAACTGATAAAAATAACTTACTTCAAGTTGAG
ATTGATTTAGATGCCTAA