Detailed information of TA system TA075923

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3367831..3368566
• Replicon: chromosome
• Accession: NZ_CP021054
• Organism: Methylorubrum zatmanii strain PSBB041

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: B2G69_RS26670
• Protein ID: WP_197707620.1
• Coordinates: 3367831..3368268 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1W6RKV4
• Locus tag: B2G69_RS15785
• Protein ID: WP_056462529.1
• Coordinates: 3368282..3368566 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B2G69_RS15765	3363385..3363915	+	531	WP_012253214.1	translation initiation factor IF-3	-
B2G69_RS15770	3364107..3366443	-	2337	WP_056506981.1	amylo-alpha-1,6-glucosidase	-
B2G69_RS15775	3366602..3367735	-	1134	WP_015822208.1	glycosyltransferase family 4 protein	-
B2G69_RS26670	3367831..3368268	+	438	WP_197707620.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B2G69_RS15785	3368282..3368566	+	285	WP_056462529.1	HigA family addiction module antidote protein	Antitoxin
B2G69_RS15790	3368760..3368963	+	204	WP_003602670.1	50S ribosomal protein L35	-
B2G69_RS15795	3369016..3369381	+	366	WP_003602669.1	50S ribosomal protein L20	-
B2G69_RS15800	3369566..3370645	+	1080	WP_015950513.1	phenylalanine--tRNA ligase subunit alpha	-
B2G69_RS15805	3370710..3373132	+	2423	Protein_3079	phenylalanine--tRNA ligase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (53-145)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-79)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 16455.52 Da
• Isoelectric Point: 7.6479

>T75923 WP_197707620.1 NZ_CP021054:3367831-3368268 [Methylorubrum zatmanii]
MVPPRAWGERQSLQPHGIPVFHICWPATQAGSRGCGCRASDGIVRCPYNDDLIILSYRNRDTEALDVHGVCHRRWRSFQA
AAGRKLDMLNAASVVSDLRSPPGNRLEKLSGDREGQWSIRINDQWRICFRWDGSGPEDVEIVDYH

Nucleotide

• Length: 438 bp

>T75923 NZ_CP021054:3367831-3368268 [Methylorubrum zatmanii]
ATGGTTCCGCCCCGCGCCTGGGGCGAGCGGCAATCTTTGCAACCCCACGGAATACCAGTTTTCCACATATGTTGGCCGGC
CACGCAGGCCGGATCGCGCGGGTGCGGTTGCAGAGCATCAGACGGTATTGTACGCTGTCCGTACAATGACGACCTCATAA
TCCTCAGCTACCGTAACAGAGACACCGAAGCGCTGGACGTGCACGGTGTGTGCCATCGCCGCTGGCGATCCTTTCAAGCT
GCGGCCGGGCGCAAGCTCGATATGCTGAACGCCGCCTCTGTCGTGAGCGACCTGCGCAGCCCGCCCGGCAACCGTCTGGA
GAAGCTGTCGGGTGATCGCGAGGGCCAGTGGAGCATCCGCATCAACGATCAATGGCGCATCTGCTTCCGATGGGACGGTT
CGGGGCCCGAGGATGTCGAGATTGTCGATTATCACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10570.11 Da
• Isoelectric Point: 7.2787

>AT75923 WP_056462529.1 NZ_CP021054:3368282-3368566 [Methylorubrum zatmanii]
MPRIRSHPGEILLEEYLRPLEMSANALAKAIEVPANRISELLRGRRGMTADTACRLGRFFGTTPEFWMSLQTAHDLSRAV
AEHDYSTLRALESA

Nucleotide

• Length: 285 bp

>AT75923 NZ_CP021054:3368282-3368566 [Methylorubrum zatmanii]
ATGCCTCGCATTAGATCTCATCCGGGCGAGATCCTGCTCGAAGAATACCTTCGACCCCTGGAAATGAGCGCCAACGCTCT
GGCGAAGGCGATCGAGGTGCCAGCCAACCGCATCAGCGAACTTCTGCGCGGTCGCCGCGGGATGACCGCCGACACGGCCT
GCCGCCTCGGCCGGTTCTTCGGGACCACGCCGGAATTCTGGATGAGCCTCCAGACCGCCCACGATCTGTCGAGGGCCGTC
GCGGAGCACGACTATTCCACCTTGCGTGCTCTCGAAAGCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1W6RKV4