Detailed information of TA system TA075741

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3656134..3656723
• Replicon: chromosome
• Accession: NZ_CP020942
• Organism: Xanthomonas oryzae pv. oryzae strain PXO61

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: B9W05_RS17975
• Protein ID: WP_012443754.1
• Coordinates: 3656442..3656723 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B9W05_RS17970
• Protein ID: WP_011260746.1
• Coordinates: 3656134..3656424 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9W05_RS17930 (B9W05_17665)	3651288..3651896	-	609	WP_011409746.1	DUF4123 domain-containing protein	-
B9W05_RS17935 (B9W05_17670)	3651899..3652321	-	423	WP_042465811.1	PAAR domain-containing protein	-
B9W05_RS17940 (B9W05_17675)	3652449..3652775	-	327	WP_228329596.1	hypothetical protein	-
B9W05_RS17950 (B9W05_17685)	3653030..3653362	+	333	WP_011260743.1	hypothetical protein	-
B9W05_RS25740	3653522..3653709	+	188	Protein_3263	hypothetical protein	-
B9W05_RS17960 (B9W05_17690)	3653812..3655206	-	1395	WP_027703652.1	type III secretion system effector XopQ	-
B9W05_RS17970 (B9W05_17700)	3656134..3656424	-	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
B9W05_RS17975 (B9W05_17705)	3656442..3656723	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B9W05_RS17980 (B9W05_17710)	3656818..3659070	-	2253	WP_069960050.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T75741 WP_012443754.1 NZ_CP020942:c3656723-3656442 [Xanthomonas oryzae pv. oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T75741 NZ_CP020942:c3656723-3656442 [Xanthomonas oryzae pv. oryzae]
GTGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCACATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCGGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT75741 WP_011260746.1 NZ_CP020942:c3656424-3656134 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT75741 NZ_CP020942:c3656424-3656134 [Xanthomonas oryzae pv. oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAATGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5