Detailed information of TA system TA075734

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 103316..103742
• Replicon: plasmid unnamed1
• Accession: NZ_CP020934
• Organism: Escherichia coli strain HB-Coli0

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: B9W17_RS24400
• Protein ID: WP_001312861.1
• Coordinates: 103316..103474 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	103518..103742 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9W17_RS24365	98690..99379	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
B9W17_RS24370	99566..99949	-	384	WP_001151524.1	relaxosome protein TraM	-
B9W17_RS24375	100270..100872	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
B9W17_RS24380	101169..101990	-	822	WP_001234426.1	DUF945 domain-containing protein	-
B9W17_RS24385	102108..102395	-	288	WP_000107535.1	hypothetical protein	-
B9W17_RS24400	103316..103474	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	103518..103742	-	225	NuclAT_0	-	Antitoxin
-	103518..103742	-	225	NuclAT_0	-	Antitoxin
-	103518..103742	-	225	NuclAT_0	-	Antitoxin
-	103518..103742	-	225	NuclAT_0	-	Antitoxin
B9W17_RS25265	103554..103742	+	189	WP_001299721.1	hypothetical protein	-
B9W17_RS24405	103754..104473	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
B9W17_RS24410	104470..104904	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
B9W17_RS24415	104959..105156	-	198	Protein_115	hypothetical protein	-
B9W17_RS24420	105184..105417	-	234	WP_000005987.1	DUF905 family protein	-
B9W17_RS24425	105485..106024	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
B9W17_RS25380	106050..106256	-	207	WP_000275856.1	hypothetical protein	-
B9W17_RS24450	107395..108366	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	oqxA / oqxB / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..151137	151137

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T75734 WP_001312861.1 NZ_CP020934:c103474-103316 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T75734 NZ_CP020934:c103474-103316 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT75734 NZ_CP020934:c103742-103518 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin