Detailed information of TA system TA075729

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 64987..65241
• Replicon: plasmid unnamed1
• Accession: NZ_CP020934
• Organism: Escherichia coli strain HB-Coli0

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: B9W17_RS24165
• Protein ID: WP_001351576.1
• Coordinates: 64987..65193 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	65180..65241 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9W17_RS24120	60539..60940	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
B9W17_RS24125	60873..61130	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
B9W17_RS24130	61223..61876	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
B9W17_RS25260	61974..62114	-	141	WP_001333237.1	hypothetical protein	-
B9W17_RS24140	62815..63672	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
B9W17_RS24145	63665..64147	-	483	WP_181726436.1	hypothetical protein	-
B9W17_RS24150	64140..64214	-	75	WP_001442103.1	RepA leader peptide Tap	-
B9W17_RS24160	64446..64703	-	258	WP_000083845.1	replication regulatory protein RepA	-
B9W17_RS24165	64987..65193	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	65180..65241	+	62	NuclAT_1	-	Antitoxin
-	65180..65241	+	62	NuclAT_1	-	Antitoxin
-	65180..65241	+	62	NuclAT_1	-	Antitoxin
-	65180..65241	+	62	NuclAT_1	-	Antitoxin
B9W17_RS25375	65497..65571	-	75	Protein_68	endonuclease	-
B9W17_RS24175	65817..66029	-	213	WP_001299730.1	ANR family transcriptional regulator	-
B9W17_RS24180	66165..66725	-	561	WP_000139315.1	fertility inhibition protein FinO	-
B9W17_RS24185	66828..67688	-	861	WP_000704514.1	alpha/beta hydrolase	-
B9W17_RS24190	67747..68493	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	oqxA / oqxB / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..151137	151137

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T75729 WP_001351576.1 NZ_CP020934:c65193-64987 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T75729 NZ_CP020934:c65193-64987 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT75729 NZ_CP020934:65180-65241 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin