Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2346584..2347086 Replicon chromosome
Accession NZ_CP020933
Organism Escherichia coli strain HB-Coli0

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag B9W17_RS11350 Protein ID WP_000767829.1
Coordinates 2346832..2347086 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag B9W17_RS11345 Protein ID WP_001259255.1
Coordinates 2346584..2346835 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9W17_RS11320 2341762..2342829 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
B9W17_RS11325 2342829..2343899 - 1071 WP_000108950.1 histidinol-phosphate transaminase -
B9W17_RS11330 2343896..2345200 - 1305 WP_001499066.1 histidinol dehydrogenase -
B9W17_RS11335 2345206..2346105 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
B9W17_RS11340 2346251..2346301 - 51 WP_001364200.1 his operon leader peptide -
B9W17_RS11345 2346584..2346835 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
B9W17_RS11350 2346832..2347086 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
B9W17_RS11355 2347169..2347993 + 825 WP_000754749.1 SDR family oxidoreductase -
B9W17_RS11360 2348039..2348968 + 930 WP_000803351.1 LysR family transcriptional regulator -
B9W17_RS11365 2349183..2349245 + 63 WP_010723108.1 membrane protein YoeI -
B9W17_RS11370 2349235..2350593 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
B9W17_RS11375 2350772..2351830 + 1059 WP_023568741.1 transport protein YeeE -
B9W17_RS11380 2351844..2352071 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T75715 WP_000767829.1 NZ_CP020933:2346832-2347086 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T75715 NZ_CP020933:2346832-2347086 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT75715 WP_001259255.1 NZ_CP020933:2346584-2346835 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT75715 NZ_CP020933:2346584-2346835 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References