Detailed information of TA system TA075707

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 294332..294934
• Replicon: chromosome
• Accession: NZ_CP020933
• Organism: Escherichia coli strain HB-Coli0

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: B9W17_RS01345
• Protein ID: WP_000897305.1
• Coordinates: 294623..294934 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B9W17_RS01340
• Protein ID: WP_000356397.1
• Coordinates: 294332..294622 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9W17_RS01315	290258..291160	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
B9W17_RS01320	291157..291792	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
B9W17_RS01325	291789..292718	+	930	WP_001749031.1	formate dehydrogenase accessory protein FdhE	-
B9W17_RS01330	293048..293290	-	243	WP_001086388.1	hypothetical protein	-
B9W17_RS01335	293509..293727	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
B9W17_RS01340	294332..294622	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
B9W17_RS01345	294623..294934	-	312	WP_000897305.1	hypothetical protein	Toxin
B9W17_RS01350	295163..296071	+	909	WP_001162704.1	alpha/beta hydrolase	-
B9W17_RS01355	296135..297076	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
B9W17_RS01360	297121..297558	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
B9W17_RS01365	297555..298427	-	873	WP_000920762.1	virulence factor BrkB family protein	-
B9W17_RS01370	298421..299020	-	600	WP_001295269.1	glucose-1-phosphatase	-
B9W17_RS01375	299119..299904	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T75707 WP_000897305.1 NZ_CP020933:c294934-294623 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T75707 NZ_CP020933:c294934-294623 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT75707 WP_000356397.1 NZ_CP020933:c294622-294332 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT75707 NZ_CP020933:c294622-294332 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7