Detailed information of TA system TA075700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2734730..2735301
• Replicon: chromosome
• Accession: NZ_CP020931
• Organism: Marinobacter salarius strain SMR5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W5YPU9
• Locus tag: MARSALSMR5_RS12490
• Protein ID: WP_041336503.1
• Coordinates: 2735023..2735301 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MARSALSMR5_RS12485
• Protein ID: WP_023012140.1
• Coordinates: 2734730..2735023 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MARSALSMR5_RS12465	2729833..2731236	-	1404	WP_085681033.1	hypothetical protein	-
MARSALSMR5_RS12470	2731612..2733693	+	2082	WP_085681035.1	bifunctional diguanylate cyclase/phosphodiesterase	-
MARSALSMR5_RS12475	2733751..2734227	-	477	WP_198324942.1	nucleoside deaminase	-
MARSALSMR5_RS12480	2734311..2734637	-	327	WP_036206826.1	DUF2834 domain-containing protein	-
MARSALSMR5_RS12485	2734730..2735023	-	294	WP_023012140.1	HigA family addiction module antidote protein	Antitoxin
MARSALSMR5_RS12490	2735023..2735301	-	279	WP_041336503.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MARSALSMR5_RS12495	2735450..2736181	-	732	WP_157665610.1	hypothetical protein	-
MARSALSMR5_RS12505	2736907..2737389	-	483	WP_085681043.1	hypothetical protein	-
MARSALSMR5_RS12510	2737465..2737887	-	423	WP_085681045.1	hypothetical protein	-
MARSALSMR5_RS12515	2737991..2738542	-	552	WP_157665611.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2727341..2747074	19733

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10833.32 Da
• Isoelectric Point: 10.0576

>T75700 WP_041336503.1 NZ_CP020931:c2735301-2735023 [Marinobacter salarius]
MIKSFRHKGLKRFYSTGHTSGIQPDHAKRLRMQLAALDTATSMEDMDIPGFRLHPLKSKDKGRWSIRVNGNWRMTFEFQD
GNAYILDYEDYH

Nucleotide

• Length: 279 bp

>T75700 NZ_CP020931:c2735301-2735023 [Marinobacter salarius]
ATGATTAAATCATTCCGTCACAAAGGACTGAAGCGGTTCTACTCGACCGGCCACACGTCTGGCATACAACCCGACCATGC
CAAGAGGCTGCGTATGCAGCTGGCTGCTCTGGATACGGCGACTTCAATGGAAGACATGGATATCCCTGGTTTTCGGCTTC
ATCCATTGAAGAGCAAAGACAAAGGCCGCTGGTCGATTAGGGTTAACGGAAATTGGCGCATGACGTTCGAATTTCAGGAC
GGCAACGCCTACATTCTTGATTATGAGGATTATCACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10770.33 Da
• Isoelectric Point: 6.2137

>AT75700 WP_023012140.1 NZ_CP020931:c2735023-2734730 [Marinobacter salarius]
MTMHNPPHPGEFIREVYLEPFGISSRQLASSLGVSPSTLSRLLKGDSGISPEMSLRLSKVLGRTPESWLAMQDMYDLWVA
RGTVNLDGIHRLDFEAA

Nucleotide

• Length: 294 bp

>AT75700 NZ_CP020931:c2735023-2734730 [Marinobacter salarius]
ATGACTATGCATAATCCGCCGCATCCTGGTGAATTCATACGGGAGGTGTATCTGGAGCCTTTCGGTATCAGTTCCCGTCA
GCTTGCGTCCAGCCTGGGGGTCTCGCCCTCCACATTGTCTCGACTGCTAAAAGGGGATAGTGGTATTAGCCCGGAAATGT
CGCTTCGGCTATCCAAGGTTCTCGGGCGTACTCCTGAGAGCTGGTTGGCGATGCAAGATATGTATGACCTATGGGTGGCT
CGCGGCACAGTCAATCTGGATGGTATTCATCGCTTGGACTTCGAAGCAGCTTAG

Structures

Toxin

• AlphaFold DB: W5YPU9