Detailed information of TA system TA075643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1943872..1944476
• Replicon: chromosome
• Accession: NZ_CP020894
• Organism: Legionella longbeachae strain F1157CHC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: B0B39_RS08530
• Protein ID: WP_085662369.1
• Coordinates: 1944165..1944476 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0W0SK75
• Locus tag: B0B39_RS08525
• Protein ID: WP_058441751.1
• Coordinates: 1943872..1944162 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0B39_RS08500	1939309..1940619	-	1311	WP_085662366.1	hypothetical protein	-
B0B39_RS08505	1940916..1941788	-	873	WP_085662367.1	helix-turn-helix transcriptional regulator	-
B0B39_RS08510	1942087..1942407	-	321	WP_003631575.1	helix-turn-helix domain-containing protein	-
B0B39_RS08515	1942397..1942696	-	300	WP_003631574.1	hypothetical protein	-
B0B39_RS18750	1942749..1942910	-	162	WP_003631573.1	hypothetical protein	-
B0B39_RS08520	1942962..1943393	-	432	WP_003631571.1	hypothetical protein	-
B0B39_RS08525	1943872..1944162	-	291	WP_058441751.1	helix-turn-helix domain-containing protein	Antitoxin
B0B39_RS08530	1944165..1944476	-	312	WP_085662369.1	hypothetical protein	Toxin
B0B39_RS08535	1944717..1945136	+	420	WP_085662371.1	hypothetical protein	-
B0B39_RS08540	1945292..1946101	+	810	WP_085662373.1	hypothetical protein	-
B0B39_RS08545	1946497..1946931	-	435	WP_085662375.1	type II toxin-antitoxin system PemK/MazF family toxin	-
B0B39_RS08550	1947118..1947816	-	699	WP_085662376.1	hypothetical protein	-
B0B39_RS08555	1947866..1948414	-	549	WP_085662378.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12001.74 Da
• Isoelectric Point: 6.3489

>T75643 WP_085662369.1 NZ_CP020894:c1944476-1944165 [Legionella longbeachae]
MIFIETPIFTEDVKELLSDEEYADFQDYLADNPLAGDVIQQTGGLRKVRWSSQGKGKRGGVRIIYYYVTPASQIRLLLIY
KKGIQDDLTTDQKKMLRQLNERW

Nucleotide

• Length: 312 bp

>T75643 NZ_CP020894:c1944476-1944165 [Legionella longbeachae]
ATGATTTTTATTGAGACTCCAATTTTTACGGAAGATGTGAAAGAATTATTATCCGATGAGGAGTATGCTGATTTTCAAGA
TTATCTTGCTGATAATCCTTTAGCTGGAGACGTAATTCAGCAAACCGGTGGACTACGAAAGGTTCGATGGTCCTCTCAAG
GCAAAGGAAAACGAGGCGGTGTTAGAATTATTTATTATTATGTAACTCCTGCTTCACAAATAAGACTCTTACTTATTTAT
AAAAAAGGCATTCAAGATGATCTGACTACAGATCAAAAGAAAATGTTACGGCAATTAAATGAACGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 11028.73 Da
• Isoelectric Point: 8.0670

>AT75643 WP_058441751.1 NZ_CP020894:c1944162-1943872 [Legionella longbeachae]
MDKKLFERLYESMTQMNEIIDGERVPSREFVVDAVHVKEIRKKTGLTQEKFCHLIDVNIGTLRNWEQGRREPTGPAKALL
RAINKDPEHVLAALAS

Nucleotide

• Length: 291 bp

>AT75643 NZ_CP020894:c1944162-1943872 [Legionella longbeachae]
ATGGATAAAAAATTATTTGAACGATTATACGAAAGTATGACTCAGATGAACGAAATTATTGATGGTGAGCGCGTTCCTTC
ACGCGAGTTCGTAGTAGATGCTGTTCATGTAAAAGAAATTAGAAAAAAAACAGGACTAACCCAAGAAAAATTTTGTCATT
TAATTGATGTAAATATTGGAACGTTAAGAAATTGGGAACAGGGGAGAAGAGAGCCAACAGGTCCCGCAAAAGCCCTTCTT
CGTGCAATAAATAAAGATCCTGAGCATGTATTAGCAGCATTAGCTAGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0W0SK75