75560

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1671091..1671688 Replicon chromosome
Accession NZ_CP020853
Organism Klebsiella pneumoniae strain KPN528

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag B8F96_RS08115 Protein ID WP_004142563.1
Coordinates 1671091..1671408 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag B8F96_RS08120 Protein ID WP_004142561.1
Coordinates 1671401..1671688 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B8F96_RS08095 1666794..1668221 - 1428 WP_004176980.1 MFS transporter -
B8F96_RS08100 1668330..1669196 + 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
B8F96_RS08110 1669863..1670906 + 1044 WP_004178895.1 DUF2157 domain-containing protein -
B8F96_RS08115 1671091..1671408 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B8F96_RS08120 1671401..1671688 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
B8F96_RS08125 1671956..1672606 - 651 Protein_1506 oxygen-insensitive NAD(P)H nitroreductase -
B8F96_RS08130 1672726..1673094 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
B8F96_RS08135 1673094..1673675 - 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
B8F96_RS08140 1673855..1674970 + 1116 WP_020316605.1 MBL fold metallo-hydrolase -
B8F96_RS08145 1675001..1675342 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
B8F96_RS08150 1675360..1675608 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T75560 WP_004142563.1 NZ_CP020853:1671091-1671408 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T75560 NZ_CP020853:1671091-1671408 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT75560 WP_004142561.1 NZ_CP020853:1671401-1671688 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT75560 NZ_CP020853:1671401-1671688 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References