Detailed information of TA system TA075548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3867719..3868316
• Replicon: chromosome
• Accession: NZ_CP020847
• Organism: Klebsiella variicola strain KPN1481

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: B8O08_RS19040
• Protein ID: WP_012542526.1
• Coordinates: 3867719..3868036 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B8O08_RS19045
• Protein ID: WP_012542525.1
• Coordinates: 3868029..3868316 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B8O08_RS19020	3863559..3864986	-	1428	WP_023297179.1	MFS transporter	-
B8O08_RS19025	3865095..3865961	+	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
B8O08_RS19035	3866491..3867534	+	1044	WP_016160437.1	DUF2157 domain-containing protein	-
B8O08_RS19040	3867719..3868036	+	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B8O08_RS19045	3868029..3868316	+	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
B8O08_RS19050	3868752..3869405	-	654	WP_012968755.1	oxygen-insensitive NAD(P)H nitroreductase	-
B8O08_RS19055	3869525..3869893	-	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
B8O08_RS19060	3869893..3870474	-	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
B8O08_RS19065	3870654..3871769	+	1116	WP_162499993.1	MBL fold metallo-hydrolase	-
B8O08_RS19070	3871800..3872141	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
B8O08_RS19075	3872158..3872406	-	249	WP_008805539.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T75548 WP_012542526.1 NZ_CP020847:3867719-3868036 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T75548 NZ_CP020847:3867719-3868036 [Klebsiella variicola]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT75548 WP_012542525.1 NZ_CP020847:3868029-3868316 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT75548 NZ_CP020847:3868029-3868316 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2