Detailed information of TA system TA075527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4652051..4652648
• Replicon: chromosome
• Accession: NZ_CP020841
• Organism: Klebsiella pneumoniae strain KPN1482

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: B8O09_RS23490
• Protein ID: WP_004893639.1
• Coordinates: 4652051..4652368 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: B8O09_RS23495
• Protein ID: WP_004142561.1
• Coordinates: 4652361..4652648 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B8O09_RS23470	4647751..4649178	-	1428	WP_004176980.1	MFS transporter	-
B8O09_RS23475	4649287..4650153	+	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
B8O09_RS23485	4650823..4651866	+	1044	WP_023279204.1	DUF2157 domain-containing protein	-
B8O09_RS23490	4652051..4652368	+	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B8O09_RS23495	4652361..4652648	+	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
B8O09_RS23500	4652786..4653439	-	654	Protein_4501	oxygen-insensitive NAD(P)H nitroreductase	-
B8O09_RS23505	4653559..4653927	-	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
B8O09_RS23510	4653927..4654508	-	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
B8O09_RS23515	4654688..4655803	+	1116	WP_074180844.1	MBL fold metallo-hydrolase	-
B8O09_RS23520	4655834..4656175	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
B8O09_RS23525	4656193..4656441	-	249	WP_002893037.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T75527 WP_004893639.1 NZ_CP020841:4652051-4652368 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T75527 NZ_CP020841:4652051-4652368 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT75527 WP_004142561.1 NZ_CP020841:4652361-4652648 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT75527 NZ_CP020841:4652361-4652648 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACGTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1